Effects of Organic Solvents on Streptomyces griseus Protease I with Transfer Action
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概要
- 論文の詳細を見る
The protease I from Streptomyces griseus var.alcalophilus strongly catalyzed the transfer reaction forming hydroxamic acids of various amino acids, especially aromatic and hydrophobic amino acids. Dimethyl sulfoxide (DMSO), 1,4-butane diol, and glycerol protected this enzyme from heat deaturation. Moreover, only DMSO strongly activated the transfer action of protease I and the velocity of reaction was accelerated three times in the presence of 50% DMSO. The yield of transfer product strongly depended on reaction time and the concentrations of enzyme and substrates in the presence of DMSO. The velocity of the transfer reaction was faster than that of the hydrolysis reaction under the conditions of 0.1 _M Phe-OEt, 0.75_M NH_2OH, and 50% DMSO.
- 社団法人日本農芸化学会の論文
- 1992-06-23
著者
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TOMINAGA Yoshio
Osaka Municipal Technical Research Institute
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Muro Tetsuo
Osaka Municipal Technical Research lnstitute
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UENO Michiyo
Industrial Technology Center of Fukui Prefecture
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Muro Tetsuo
Osaka Municipal Technical Research Institute
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