Penicillium abeanum Lipase : Purification, Characterization, and Its Use for Docosahexaenoic Acid Enrichment of Tuna Oil
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概要
- 論文の詳細を見る
Penicillium abeanum lipase was purified to homogeneity as judged by non-denaturing and denaturing polyacrylamide gel electrophoresis. The enzyme is a monomeric protein with a molecular mass of approximately 28 kDa and a pI of 3.4. The optimal pH at 25°C and the optimal temperature at pH 7.5 were 7-8,and 25-30°C, respectivey. The enzyme hydrolyzes the 1- and 3-position ester bonds at nearly nine times the rate of cleavage at the 2-position. The enzyme showed lower activities towards ester bonds of polyunsaturated fatty acids as compared with those of other acids, and was proved effective for increasing the concentration of docosahexaenoic acid (22 : 6) in tuna oil.
- 社団法人日本生物工学会の論文
- 1996-11-25
著者
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Tominaga Y
Dep. Of Applied Biochemistry And Food Sci. Saga Univ.
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Yanagita Teruyoshi
Lab. Of Nutrition Biochemistry Dep. Of Applied Biochemistry And Food Sci. Saga Univ.
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Shimada Y
Department Of Biochemistry Osaka Municipal Technical Research Institute
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Shimada Y
Department Of Physics Chuo University:tecmo
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Shimada Yuji
Osaka Municipal Technical Research Institute
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Takada Nobuo
Kaneka Corporation
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NAGAO Toshihiro
Osaka Municipal Technical Research Institute
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SUGIHARA Akio
Faculty of Engineering, Tokushima Bunri University
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SUGIHARA Akio
Osaka Municipal Technical Research Institute
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TOMINAGA Yoshio
Osaka Municipal Technical Research Institute
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TAKADA NOBUO
Katayama Chemical Inc.,
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Yanagita Teruyoshi
Dep. Of Applied Biochemistry And Food Sci. Saga Univ.
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Tominaga Yoshio
The Organ Transplant Center Nagoya Second Red Cross Hospital
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Sugihara A
Faculty Of Engineering Tokushima Bunri University
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Nagao Toshihiro
Biomaterials And Commodity Chemicals Res. Div. Osaka Municipal Technical Res. Inst.
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Nagao Toshihiro
Department Of Biochemistry Osaka Municipal Technical Research Institute
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Takada Nobuo
Katayama Chemical Inc.
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