A Novel Human Hepatoblastoma Cell Line (HuH-6KK) with Rapid Growth in Serum-free Medium without Extracellular Matrix(Biological Chemistry)
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概要
- 論文の詳細を見る
A novel human hepatoblastoma cell line (HuH-6KK) with a high growth rate in a serum-free medium without extracellular matrix was developed from an original one, HuH-6 c!5 (HuH). The original HuH cells (38 passages) did not proliferate well in RPMI1640 medium containing 20% fetal calf serum (FCS). The HuH cells (HuH-6KK) with a high growth rate were selected by culturing them in an enriched RDF containing 20% FCS and 0.01% mucous polysaccharide (spirulinan) isolated from a blue-green alga, Spirulina subsalsa. The HuH-6KK cells showed a rapid growth in serum-free eRDF medium containing insulin, transferrin, ethanolamine, and selenite (eRDF-ITES medium) without fibronectin. The proliferation of the original HuH cells was also observed in the eRDF-ITES medium, but the growth was slow compared with the HuH-6KK cells. In the medium without ITES, the growth of the HuH-6KK and original HuH cells was slow. Among the ITES ingredients, insulin promoted the growth of HuH-6KK cells the most.
- 社団法人日本農芸化学会の論文
- 1990-10-23
著者
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Kong Z‐l
National Taiwan Ocean Univ. Keelung Twn
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SHINOHARA Kazuki
National Food Research Institute, Ministry of Agriculture, Forestry, and Fisheries, Tsukuba
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Murakami Hiroki
Graduate School of Genetic Resources Technology, Kyushu University
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KONG Zwe-Ling
Institute of Food Science and Technology, Faculty of Agriculture, Kyusyu University
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Murakami H
Kyushu Univ. Fukuoka Jpn
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Murakami H
Graduate School Of Genetic Resources Technology Kyushu University
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Kong Zwe-ling
Institute Of Food Science And Technology Faculty Of Agriculture Kyushu University
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Kong Zwe-ling
Institute Of Food Chemistry Department Of Food Science And Technology Faculty Of Agriculture Kyushu
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Murakami H
Osaka Municipal Technical Res. Inst.
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Murakami Hiroki
Graduate School Of Genetic Resources Technology Kyushu University
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Murakami Hiroki
Graduate School Of Genetic Resources Technology Faculty Of Agriculture Kyushu University
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Murakami H
Graduate School Of Genetic Resources Technology Faculty Of Agriculture Kyushu University
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NAGAMINE Kenichi
Nichirei Research Institute
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SHIRAISHI Masato
National Food Research Institute
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Shinohara K
National Food Research Institute
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Shinohara Kazuki
National Food Res. Inst. Ibaraki Jpn
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Shinohara Kazuki
National Food Research Institute
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Shinohara Kazuki
Laboratory Of Food Chemistry Faculty Of Agriculture Kyushu University
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Nagamine Kenichi
Department Of Food Science And Technology Faculty Of Agriculture Kyushu University
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Shinohara Kazuki
Food Chemistry Institute Faculty Of Agriculture Kyushu University
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