Identification of an N-acetylcysteine conjugate in the urine after oral administration of 2,4-dichloro-1-nitrobenzene to rats
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概要
- 論文の詳細を見る
Oral administration of 2,4-dichloro-1-nitrobenzene (2,4-DCNB) causes kidney tumors in the rat. The objective of the present study was to identify the chemical structure of 2,4-DCNB metabolites in urine. Urine from 2,4-DCNB fed rats was more yellow than urine from control rats, exhibiting a broad UV-spectrum around a peak wavelength of 360nm; the control urine did not have an absorbance. The yellow component was extracted and analyzed. The optical properties of the yellow component were the same as the N-acetylcysteine conjugate of 1,4-dichloro-2-nitrobenzene (1,4-DCNB): 1,4-DCNB is secreted in urine as an N-acetylcysteine conjugate. LC-MS/MS analyses of this yellow component demonstrated its chemical structure to be the N-acetylcysteine conjugate of 2,4-DCNB. Nuclear overhauser effect and LC-MS/MS analyses revealed the structural isomer of this 2,4-DCNB metabolite as N-acetyl-S-(5-chloro-2-nitrophenyl)-L-cysteine. We also discuss the possibility that the N-acetylcysteine conjugate identified in this study plays a role as a proximate carcinogen in the formation of kidney tumors.
- 日本トキシコロジー学会の論文
- 2009-04-01
著者
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Fukushima S
Japan Bioassay Research Center Japan Industrial Safety And Health Association
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Fukushima Shoji
Japan Bioassay Research Center, Japan Industrial Safety and Health Association
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Yamamoto Seigo
Japan Bioassay Research Center Japan Industrial Safety And Health Association
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Take Makoto
Japan Bioassay Research Center, Japan Industrial Safety and Health Association
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Ohnishi Makoto
Japan Bioassay Research Center, Japan Industrial Safety and Health Association
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Yajima Hirofumi
Department of Applied Chemistry, Faculty of Science, Tokyo University of science
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Take Makoto
Japan Bioassay Research Center Japan Industrial Safety And Health Association
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Take Makoto
Japan Bioassay Laboratory
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Ohnishi Makoto
Japan Bioassay Res. Center Japan Industrial Safety And Health Assoc.
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Ohnishi Makoto
Japan Bioassay Research Center Japan Industrial Safety And Health Association
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Yajima H
Department Of Applied Chemistry Faculty Of Science Tokyo University Of Science
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Yajima Hirofumi
Department Of Applied Chemistry Faculty Of Science Tokyo University Of Science
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Yajima Hirofumi
Department Of Applied Chemistry Faculty Of Science Science University Of Tokyo
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Fujiwara Yushi
Department Of Gastroenterological Surgery Osaka City University Graduate School Of Medicine
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Fukushima Shoji
Japan Bioassay Research Center Japan Industrial Safety And Health Association
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Fukushima Shoji
中央労働災害防止協会日本バイオアッセイ研究センター
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Fukushima Shoji
Japan Bioassay Research Center
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Fukushima Shoji
Japan Bioassay Res. Center Japan Industrial Safety And Health Assoc.
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