Purification and Characterization of Three Extracellular Protopectinases with Poly-galacturonase Activities from Trichosporon penicillatum
スポンサーリンク
概要
- 論文の詳細を見る
In a culture filtrate of Trichosporon penicillatum B2, which is a γ-ray irradiation mutant induced from T. penicillatum SNO3, we found three kinds of pectin-releasing enzymes, protopectinases SE1, SE2, and SE3, that have endo-polygalacturonase activity. These enzymes were purified to homogeneity with cation-exchange and size exclusion chromatographies. The major PPase in the culture filtrate was PPase SE1, which accounted for 75% of total activities in the culture filtrate, and the two others were 0.15% (PPase SE2) and 0.007% (PPase SE3). Their molecular masses were approximdtely 41, 41 and 42 kDa on SDS-PAGE, respectively. They had similar enzymatic propertied but different PPase activity and pH- and thermo-stability. Antibody against PPase S, which is produced by strain SNO3, inhibited the activities of PPase SE1, SE2, and SE3. However PPase SE1 was completely inhibited by treatment with the anti-PPase S antibody, but the activities of PPases SE2 and SE3 remained at 20 and 50% of the original activity, respectively.
- 社団法人日本農芸化学会の論文
- 1996-04-23
著者
-
Kishida Masao
Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture University
-
SAKAI TAKUO
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
-
Kishida Masao
Department Of Applied Biochemistry College Of Agriculture Osaka Prefecture University
-
Sakai Takuo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
-
Sakai Takuo
Department Of Applied Biochemistry College Of Agriculture Osaka Prefecture University
-
IGUCHI Ken-ichi
Department of Applied Biochemistry, College of Agriculture, Osaka Prefecture University
-
Iguchi Ken-ichi
Department Of Applied Biochemistry College Of Agriculture Osaka Prefecture University
関連論文
- Purification and Characterization of a Pectin-Releasing Enzyme Produced by Kluyveromyces wickerhamii
- Chromosomal Deletion or Rearrangement in Chimeric Hybrids of Saccharomycopsis fibuligera and Saccharomyces diastaticus Obtained by Cell Fusion
- Introduction of Mitochondria into Respiratory-Deficient Mutant of Yeast and Improvement of Thermostability
- Breeding a Fermentative Yeast at High Temperature Using Protoplast Fusion
- FM-P46 Optimization of bacteriocin production by thermotolerant lactic acid bacteria, Pediococcus KKU 170, isolated from Thai local fermented food(Section VIII Fermentation and Microbial Technology)
- Production of Monoclonal Antibody against Protopectinase from Kluyveromyces wickerhamii : Studies of Enzymes Produced by Yeasts (IX).
- Cloning and Heterologous Expression of Gene Encoding A Polygalacturonase from Aspergillus awamori
- Purification and Characterization of Acid-stable Protopectinase Produced by Aspergillus awamori in Solid-state Fermentation
- Purification and Characterization of an Endo-Polygalacturonase from a Mutant of Saccharomyces cerevisiae
- Isolation and Genetic Determination of a Saccharomyces cerevisiae Mutant That Produces an Endo-Polygalacturonase
- Molecular Cloning and Expression of a Polygalacturonase Gene in Saccharomyces cerevisiae
- Molecular Cloning of the Gene Encoding Thermostable Endo-1,5-α-L-arabinase of Bacillus thermodenitrificans TS-3 and Its Expression in Bacillus subtilis(Biochemistry & Molecular Biology)
- Molecular Cloning, DNA Sequence, and Expression of the Gene Encoding for Thermostable Pectate Lyase of Thermophilic Bacillus sp. TS 47
- Purification and Characterization of Thermostable Pectate Lyase with Protopectinase Activity from Thermophilic Bacillus sp. TS 47
- Purification and Characterization of an Endo-Polygalacturonase from Aspergillus awamori
- Studies on Protopectinase-C Mode of Action : Analysis of the Chemical Structure of the Specific Substrate in Sugar Beet Protopectin and Characterization of the Enzyme Activity
- Cloning and Expression in Escherichia coli of Sucrose Phosphorylase Gene from Leuconostoc mesenteroides No. 165
- Screening for Bacteria Producing Sucrose Phosphorylase and Characterization of the Enzymes
- Cloning and Characterization of Zymomonas mobilis Genes Encoding Extracellular Levansucrase and Invertase
- Cloning and Characterization of a Pair of Genes That Stimulate the Production and Secretion of Zymomonas mobilis Extracellular Levansucrase and Invertase
- Purification and Characterization of Three Extracellular Protopectinases with Poly-galacturonase Activities from Trichosporon penicillatum
- Enantioselective Oxidation of Diols by Secondary Alcohol Dehydrogenase from Geotrichum sp. WF9101
- Degradation of Vinyl Alcohol Oligomers by Geotrichum sp. WF9101
- Isolation and Characterization of a Strain of Bacillus megaterium That Degrades Poly(vinyl alcohol)
- Purification, Characterization, and Production of Two Pectic Transeliminases with Protopectinase Activity from Bacillus subtilis
- Purification and Some Properties of a Protopectin-solubilizing Enzyme that Has Potent Activity on Sugar Beet Protopectin(Biological Chemistry)
- Isolation and Genetic Characterization of pGKL Killer-insensitive Mutants (iki) from Saccharomyces cerevisiae
- A Cold-Sensitive spo14 Mutation Affecting Ascospore Formation in the Fission Yeast Schizosaccharomyces pombe
- Purification and Characterization of Adenine Deaminase in Pseudomonas synxantha : Metabolism of nucleosides in bacteria (VIII)
- Purification, Crystallization, and Characterization of a Novel Protopectinase from Bacillus subtilis(Biological Chemistry)
- Microbial Metabolism of Nitriles in Pseudomonas sp. : Metabolism of Nitriles in Microorganisms. (VII)
- Protopectin Solubilizing Enzyme That Does Not Catalyze the Degradation of Polygalacturonic Acid(Biological Chemistry)
- Thermotolerant Bacteriocin-Producing Lactic Acid Bacteria Isolated from Thai Local Fermented Foods and Their Bacteriocin Productivity
- Use of Protoplast Fusion for the Development of Rapid Starch-fermenting Strains of Saccharomyces diastaticus
- Formation of β-Cyanoalanine by Cyanide-resistant Strain Enterobacter sp. 10-1
- Purification and Crystallization of a β-Cyanoalanineforming Enzyme from Enterobacter sp. 10-1
- Purification, Crystallization and Some Properties of Endopolygalacturonase from Kluyveromyces fragilis
- Purification and SomeProperties of a Protopectin-solubilizing Enzymefrom Galactomyces reessii Strain L
- Some Properties of a β-Cyanoalanine-forming Enzyme of Enterobacter sp. 10-1
- Purification, Crystallization and Some Properties of β-Cyano-L-alanine-degrading Enzyme in Pseudomonas sp. 13
- Possible Role of Cell Wall Mannan in the Secretion of the Protopectin-solubilizing Enzyme of Trichosporon penicillatum SNO-3
- Microbial Utilization of β-Cyano-L-alanine by Pseudomonas sp. 13
- Some Properties of Endo-polygalacturonase from Trichosporon penicillatum SNO-3
- Purification and Crystallization of a Protopectin-solubilizing Enzyme from Trichosporon penicillatum