Isolation and Nucleotide Sequence Analysis of the URA3 (orotidine 5'-phosphate decarboxylase) Gene of Kluyverimyces lactis(Microbiology & Fermentation Industry)

スポンサーリンク

概要

• 論文の詳細を見る

• A gene complementing a ura3 mutation in Saccharomyces cerevisiae was isolated from Kluyveromyces lactis. Its nucleotide sequence showed an open reading frame of 801 bp, corresponding to 267 amino acid residues. These are homologous to the coding region of URA3 gene of S. cerevisiae, 71% and 82% at the nucleotide and the amino acid levels, respectively. Although transcription started at -30 to -40 from the initiation codon ATG, which is comparable to S. cerevisiae, the regulatory sequences identified in the 5' flanking regions of URA1 and URA3 genes of S. cerevisiae are not found in the URA3 gene of K. lactis.

• 社団法人日本農芸化学会の論文
• 1988-12-23

著者

• HISHINUMA FUMIO

  Mitsubishi Kasei Institute of Life Science

• Mizutani Makoto

  Mitsubishi Kasei Institute Of Life Sciences:(present Office)faculty Of Agriculture Nagoya University

関連論文

• Optimization of the Expression of a Foreign Gene from Galactose-Inducible Promoter in a Recombinant Yeast
• Characterization of IKI1 and IKI3 Genes Conferring pGKL Killer Sensitivity on Saccharomyces cerevisiae
• Isolation and Genetic Characterization of pGKL Killer-insensitive Mutants (iki) from Saccharomyces cerevisiae
• Isolation and Nucleotide Sequence Analysis of the URA3 (orotidine 5'-phosphate decarboxylase) Gene of Kluyverimyces lactis(Microbiology & Fermentation Industry)
• Construction of a Plasmid Replicating in Both Saccharomyces cerevisiae and Kluyveromyces lactis
• Intact Cell Transformation of Saccharomyces cerevisiae by Polyethylene Glycol

スポンサーリンク