Construction of Prevotella ruminicola-Escherichia coli Shuttle Vector pRAM45 and Transformation of P.ruminicola Strains by Electroporation

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概要

• 論文の詳細を見る

• A new plasmid shuttle vector, pRAM45, was constructed from the Escherichia coli plasmid pACYC184 and the cryptic plasmid pRAM4, which was originally isolated by us from Prevotella ruminicola T31 (Ogata et al., Plasmid, 35, 91-97, 1996). The vector was electrotransformed into different P.ruminicola strains. Polymerase chain reaction and Southern hybridization experiments confirmed its integrity in P.ruminicola transformants.

• 社団法人日本生物工学会の論文
• 1999-09-25

著者

• 田島 清

  畜草研

• TAJIMA Kiyoshi

  National Institute of Livestock and Grassland Science

• Tajima Kiyoshi

  Division Of Biomaterials Department Of Oral Functional Reconstruction Kyushu Dental Collage

• Nagamine T

  Staff‐inst. Tsukuba‐shi Jpn

• Nagamine Takafumi

  農林水産先端技術産業振興センター農林水産先端技術研究所

• Nagamine Takafumi

  Staff Institute

• Nagamine Takafumi

  Rumen Microbiology Research Team Staff-institute

• Ogata K

  Staff‐inst. Tsukuba‐shi Jpn

• Ogata Koretsugu

  農林水産先端技術産業振興センター農林水産先端技術研究所

• Ogata Koretsugu

  Staff Institute

• Ogata Koretsugu

  Rumen Microbiology Research Team Staff-institute

• AMINOV ROUSTAM

  Department of Animal Sciences, University of Illinois

• TAJIMA KIYOSHI

  Rumen Microbiology Research Team, STAFF-Institute

• NAKAMURA MATSUMI

  Rumen Microbiology Research Team, STAFF-Institute

• MATSUI HIROKI

  Rumen Microbiology Research Team, STAFF-Institute

• BENNO YOSHIMI

  Rumen Microbiology Research Team, STAFF-Institute

• Nakamura Matsumi

  Rumen Microbiology Research Team Staff-institute

• Benno Yoshimi

  Microbe Division/japan Collection Of Microorganisms Riken Bio Resource Center

• Tajima K

  Division Of Biomaterials Department Of Oral Functional Reconstruction Kyushu Dental Collage

• Aminov Roustam

  Department Of Animal Sciences University Of Illinois At Urbana-champaign

• Benno Yoshimi

  Rumen Microbiology Research Team Staff-institute

• Benno Yoshimi

  The Institute Of Physical And Chemical Research

• Benno Yoshimi

  Japan Collection Of Microorganisms (jcm)

• Nakamura Mayumi

  Department Of Biotechnology The University Of Tokyo

• Benno Yoshimi

  Japan Collection Of Microorganisms Riken Bioresource Center

• Benno Y

  Microbe Division/japan Collection Of Microorganisms Riken Bio Resource Center

• Aminov R

  Mie Univ. Tsu Jpn

• Matsui H

  Rumen Microbiology Research Team Staff-institute

• Nakamura M

  Rumen Microbiology Research Team Staff-institute

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