The Effect of Protozoa on the Composition of Rumen Bacteria in Cattle Using 16S rRNA Gene Clone Libraries
スポンサーリンク
概要
- 論文の詳細を見る
The effect of the presence of protozoa on the composition of rumen bacteria was investigated in cattle. Seven castrated Holstein cattle were divided into two groups: four faunated and three unfaunated, and 16S ribosomal RNA gene (rDNA) clonal libraries were constructed. A total of 312 clones were sequenced across 1,500 bp. The 151 sequences of the faunated cattle were classified into 98 operational taxonomic units (OTUs) having at least 97% similarity. The sequences derived from the faunated cattle were classified into Firmicutes (59.7%), Bacteroidetes (34.4%), Spirochaetes (2.6%), Actinobacteria (2.0%), and Proteobacteria (1.3%). Bacteroides and Prevotella (34.4%) were the major groups in the faunated cattle. The 161 sequences in the unfaunated cattle were classified into 72 OTUs. The sequences derived from the unfaunated libraries were classified into Firmicutes (65.7%), Bacteroidetes (31.1%), Proteobacteria (1.9%), and Spirochaetes (1.2%). The Clostridium botulinum group and its relatives (36.0%) were the major groups in the unfaunated cattle.An analysis by the computer program LIBSHUFF clarified that the presence of ruminal protozoa markedly affected the composition of rumen bacteria.
- 社団法人 日本農芸化学会の論文
- 2005-03-23
著者
-
田島 清
畜草研
-
Ozutsumi Yuhei
United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology
-
TAJIMA Kiyoshi
National Institute of Livestock and Grassland Science
-
TAKENAKA Akio
National Institute of Livestock and Grassland Science
-
ITABASHI Hisao
United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology
-
竹中 昭雄
農業･食品産業技術総合研究機構畜産草地研究所
-
Takenaka A
National Institute Of Livestock And Grassland Science
-
Tajima Kiyoshi
Division Of Biomaterials Department Of Oral Functional Reconstruction Kyushu Dental Collage
-
田島 清
National Institute Of Livestock And Grassland Science
-
Itabashi Hisao
United Graduate School Of Agriculture Tokyo University Of Agriculture And Technology
-
Itabashi Hisao
東京農工大学 農学部
-
Itabashi Hisao
United Graduate School Of Agricultural Sci. Tokyo Univ. Of Agriculture And Technol.
-
Itabashi H
United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology
-
Itabashi Hisao
Department Of Animal Physiology National Institute Of Animal Industry
-
Itabashi Hisao
Faculty Of Agriculture Tokyo University Of Agriculture And Technology
-
竹中 昭雄
畜産草地研究所
-
竹中 昭雄
National Institute Of Livestock And Grassland Science
-
Tajima K
Division Of Biomaterials Department Of Oral Functional Reconstruction Kyushu Dental Collage
-
Tajima Kiyoshi
Faculty Of Regional Environmental Science Tokyo University Of Agriculture
-
Takenaka A
Graduate School Of Bioscience And Biotechnology Tokyo Institute Of Technology
-
Takenaka Akio
Department Of Life Science Faculty Of Bioscience And Biotechnology Tokyo Institute Of Tdechnology
-
Ozutsumi Yuhei
東京農工大学 連合農学研究科
-
Takenaka Akio
National Inst. Environmental Studies Ibaraki Jpn
関連論文
- オカラサイレージ、乾燥オカラ、オカラテンペの給与が肥育豚の回腸末端内容物の性状に及ぼす影響
- 濃厚洗米排水の保存性と飼料特性
- コンビニエンスストアから排出された消費期限切れ食品を主体とする発酵リキッド飼料によるブタの肥育試験
- Effects of sandblasting media and steam cleaning on bond strength of titanium-porcelain
- 米由来アルコール蒸留廃液の飼料特性評価
- The Effect of Protozoa on the Composition of Rumen Bacteria in Cattle Using 16S rRNA Gene Clone Libraries
- テンペ菌発酵トウフ粕の養豚用飼料としての利用性
- ギ酸添加による甘しょ焼酎粕の保存性改善
- PCR法による肥料中の動物由来DNAの検出
- Difference in Structure of the Gene Encoding H^+-ATPase between Streptococcus bovis and Ruminococcus albus
- Difference in Structure of the Gene Encoding H^+-ATPase between Streptococcus bovis and Ruminococcus albus
- A-19 配列特異的SSU rRNA切断法を用いたウシルーメン細菌叢の定量的解析(ルーメン・消化管生態系,口頭発表)
- Cloning, Expression, and Characterization of a Cellulase Gene from Prevotella ruminicola
- Construction of Prevotella ruminicola-Escherichia coli Shuttle Vector pRAM45 and Transformation of P.ruminicola Strains by Electroporation
- Molecular cloning, nucleotide sequence and characteristics of a xylanase gene (xynA) from Ruminococcus albus 7
- ルーメンプロトゾアが子牛の血漿ガストリン濃度に及ぼす影響
- Inhibitory effects of isoleucine and antagonism of the other branched-chain amino acids on fermentation parameters by mixed ruminal microbes in batch cultures and rumen simulating fermenters (Rusitec )
- Effects of amino nitrogen on fermentation parameters by mixed ruminal microbes when energy or nitrogen is limited
- ルーメン細菌叢に及ぼすプロトゾアの影響の分子生物学的手法による解析
- ルーメンプロトゾアの繊維分解酵素とその遺伝子解析
- Fiber Digestion by Rumen Ciliate Protozoa
- ル-メン内窒素代謝と生産性(6)窒素化合物の消化とル-メンプロトゾア
- ル-メンプロトゾアの機能と意義-7-プロトゾア構成とル-メン発酵および細菌叢との関係
- Comparative digestion of cell wall components of alfalfa hay cubes between Sika deer (Cervus nippon) and cattle
- Seasonal changes in digestibility, passage rate and rumen fermentation of alfalfa hay in sika deer (Cervus nippon) under restricted feeding
- Comparison of digestibility, passage rate and rumen fermentation between sika deer (Cervus nippon) and cattle fed alfalfa hay cubes
- Effect of cyclodextrin diallyl maleate on methane production, ruminal fermentation and microbes in vitro and in vivo
- Three-Dimensional Finite Modeling from CT Images of Tooth and Its Validation
- Electropolishing of CP Titanium and Its Alloys in an Alcoholic Solution-based Electrolyte
- Corrosion Behavior of Dental Alloys in Various Types of Electrolyzed Water
- Durability of Bactericidal Activity in Electrolyzed Neutral Water by Storage
- Application of Electrolyzed Acid Water to Sterilization of Denture Base Part 1.Examination of Sterilization Effects on Resin Plate
- Analysis of methanogens in the bovine rumen by polymerase chain reaction single-strand conformation polymorphism
- PCR Detection of DNAs of Animal Origin in Feed by Primers Based on Sequences of Short and Long Interspersed Repetitive Elements(Food & Nutrition Science)
- The Characteristics of Land-surface Spectral Reflectance in Djibouti
- Monitoring the Land Surface Changes of Djibouti Using LANDSAT Images
- Evapotranspiration and Irrigation of Zoysia matrella Merr.
- The Efficiency and Improvement of Simple Distillation Device by Solar Energy
- 太陽放射エネルギーを利用した蒸留について
- Recent Topics in Rumen Microbiology with Particular Reference to Animal Production in Japan
- NDFと脂肪含有量に富む食品製造副産物の給与が泌乳前期の乳生産に及ぼす影響
- Increase of ruminal fiber digestion by cellobiose and a twin strain of Saccharomyces cerevisiae live cells in vitro
- Effect of α-cyclodextrin-allyl isothiocyanate on ruminal microbial methane production in vitro
- Effects of medium-chain fatty acid-cyclodextrin complexes on ruminal methane production in vitro
- Crystal Structure of Eucaryotic E3, Lipoamide Dehydrogenase from Yeast^1
- Crystallization of Eukaryotic E3, Lipoamide Dehydrogenase, from Yeast, for Exhibiting X-Ray Diffraction beyond 2.5 A Resolution, and Preliminary Structure Analysis
- ル-メンプロトゾアの機能と意義-8-宿主に対するプロトゾアの機能
- A Simulation Model of Tree Architecture Development Based on Growth Response to Local Light Environment
- Effect of ruminal protozoa on fecal microbiota in cattle using terminal restriction fragment length polymorphism
- Effects of cyclodextrin-iodopropane complex on methane production, ruminal fermentation and microbes, digestibility and blood metabolites in steers
- Diversity of the Formyltetrahydrofolate Synthetase Gene (fhs), a Key Enzyme for Reductive Acetogenesis, in the Bovine Rumen
- Effect of propylene glycol and undegradable protein source on rumen fermentation, blood metabolism and milk production in lactating dairy cows
- Effect of brewer's grain on rumen fermentation, milk production and milk composition in lactating dairy cows
- Effects of the essential oil cyclodextrin complexes on ruminal methane production in vitro
- Crystallographic Studies on Damaged DNAs : III. N^4-Methoxycytosine Can Form Both Watson-Crick Type and Wobbled Base Pairs in a B-Form Duplex^1
- Structural Composition of Hammerhead Ribozymes
- N^τ-Methylhistidine Content in the Organs and Tissues of Goats(Food & Nutrition)
- Protein Quality of High-yielding Rice and Its Improvement by Supplementation of Lysine and Threonine(Food & Nutrition)
- Factors Controlling the Recruitment of Aster kantoensis (Asteraceae) : I. Breeding System and Pollination System
- Local coexistence of tree species and the dynamics of global distribution pattern along an environmental gradient : a simulation study
- Reclamation of Saline Soil in Northeastern Thailand Using a Solar-Powered Pump to Control Groundwater Levels
- Dynamics of seedling populations and tree species coexistence in a forest : a simulation study
- Crystal Structure of 3-Isopropylmalate Dehydrogenase from the Moderate Facultative Thermophile, Bacillus coagulans: Two Strategies for Thermostabilization of Protein Structures
- 酵母発酵培養物の添加が乳蛋白質生産,ル-メン発酵および血液性状に及ぼす影響
- Effects of protozoa on the antioxidant activity in the ruminal fluid and blood plasma of cattle
- Comparison of effects of spatial autocorrelation on distribution predictions of four rare plant species in the Watarase wetland
- Insights into the Structures of DNA Damaged by Hydroxyl Radical: Crystal Structures of DNA Duplexes Containing 5-Formyluracil
- Seasonal changes in herbage production and soil phosphorus contents in Japanese lawngrass (Zoysia japonica Steud.) and tall fescue (Festuca arundinacea Schreb.) pastures
- 子牛のルーメン細菌および発酵パターンに及ぼすDasytricha EpidiniumおよびEntodiniumの影響
- Culture-Independent Analysis of Fecal Microbiota in Cattle
- Diversity of the Formyltetrahydrofolate Synthetase Gene (fhs), a Key Enzyme for Reductive Acetogenesis, in the Bovine Rumen
- 散在性反復配列を用いた飼料中の動物由来DNAの検出法
- 高エネルギー飼料給与時におけるホルスタイン種育成雌牛の発育および窒素出納に及ぼす飼料中粗タンパク質および第一胃非分解性タンパク質含量の影響
- Fatty acid composition of ruminal bacteria and protozoa, and effect of defaunation on fatty acid profile in the rumen with special reference to conjugated linoleic acid in cattle
- Effect of supplementation of soy sauce oil and Ca salts of fatty acids on rumen fermentation, milk production and conjugated linoleic acid in milk of dairy cows
- Actinohivin : specific amino acid residues essential for anti-HIV activity
- 育成前期乳牛の生理および窒素・エネルギー代謝に及ぼす高温高湿の影響
- Radiocesium Distribution in the Tissues of Japanese Black Beef Heifers Fed Fallout-Contaminated Roughage Due to the Fukushima Daiichi Nuclear Power Station Accident
- 放射性セシウムを含む玄米配合飼料を給与したブタ体組織における放射性セシウム濃度
- Difference in Structure of the Gene Encoding H+-ATPase between Streptococcus bovis and Ruminococcus albus
- Crystal Structure of Eucaryotic E3, Lipoamide Dehydrogenase from Yeast.
- 飼料用米等の給与が豚肉の識別性におよぼす影響