Rapid DNA Extraction from a Bloom-forming Cyanobacterium

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概要

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• A rapid small-scale DNA extraction method is described for the toxic and/or bloom-forming cyanobacterial genus Microcystis, producing enough genomic DNA for polymerase chain reaction (PCR) amplification. PCR templates from 43 Microcystis strains were extracted and analyzed by PCR amplification. Sonication was needed for some strains before extraction of DNA using InstaGene^<TM> Matrix with heat treatment at 100℃. DNAs extracted from all strains used in this study by this method could be used as templates for PCR amplification. Depending on the apparatuses used, DNA extraction, PCR amplification and agarose gel electrophoresis analysis can be carried out on about 50 samples of Microcystis one day.

• 1999-09-30

著者

• WATANABE Masayuki

  Department of Pediatrics and Developmental Medicine, Gunma University Graduate School of Medicine

• KONDO RYUJI

  Department of Marine Bioscience, Fukui Prefectural University

• HIROISHI SHINGO

  Department of Marine Bioscience, Fukui Prefectural University

• KAGIYA Go

  Department of Radiological Sciences, Faculty of Medicine, Toyama Medical and Pharmaceutical Universi

• Kagiya Go

  Department Of Marine Bioscience Fukui Prefectural University

• Kondo R

  Department Of Marine Bioscience Fukui Prefectural University

• Kondo Ryuji

  Department Of Physics Kyusyu University

• Kondo Ryuji

  Department Of Biological Sciences University Of Essex Uk:department Of Marine Bioscience Fukui Prefe

• Hiroishi Shingo

  Department Of Marine Bioscience Fukui Prefectural University

• Hiroishi S

  Faculty Of Biotechnology Fukui Prefectural University

• Hiroishi S

  Department Of Bioscience Fukui Prefectural University

• HATA YOSHIHIKO

  Department of Marine Bioscience, Fukui Prefectural University

• Watanabe Masayuki

  Department Of Botany National Science Museum

• Hata Yoshihiko

  Department Of Marine Bioscience Fukui Prefectural University

• Watanabe Masayuki

  Department Of Biology Graduate School Of Natural Science Konan University

• Hiroishi Shingo

  Department of Bioscience, Fukui Prefectural University

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