Cryopreservation of a Myovirus Infecting the Toxin-Producing Cyanobacterium Microcystis aeruginosa

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概要

• 論文の詳細を見る

• We optimized conditions for cryopreservation of a cyanophage (Ma-LMM01) infecting the toxic cyanobacterium Microcystis aeruginosa. The quality of cryopreservation was estimated by comparing the phage titer before and after preservation at 4, -80, or -196℃ and with or without a cryoprotectant: glycerol, dimethyl sulfoxide (DMSO), or Cellbanker. Storage at 4℃ for 100 days resulted in 〜90% loss of infectivity; whereas cryopreservation at -196℃ resulted in stable preservation with or without cryoprotectant. Therefore, we established methods to stably preserve the phage, Ma-LMM01, that may be useful in further studies of cyanophages and may be used in isolating new phages.

• 日本微生物生態学会の論文

著者

• YOSHIDA Takashi

  Department of Pharmacognosy, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Oka

• NAGASAKI KEIZO

  National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency

• KASHIMA AKI

  Department of Marine Bioscience, Fukui Prefectural University

• HIROISHI SHINGO

  Department of Marine Bioscience, Fukui Prefectural University

• Takao Yoshitake

  Department Of Agriculture Kyoto University

• NAGASAKI KEIZO

  Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea

• Takashima Yukari

  Department of Marine Bioscience, Faculty of Biotechnology, Fukui Prefectural University

• Kashima Aki

  Department Of Marine Bioscience Fukui Prefectural University

• Nagasaki Keizo

  National Res. Inst. Of Fisheries And Environment Of Inland Sea Fisheries Res. Agency

• Nagasaki Keizo

  Harmful Algal Bloom Division National Research Institute Of Fisheries And Environment Of Inland Sea

• Hiroishi Shingo

  Department Of Marine Bioscience Fukui Prefectural University

• Yoshida Takashi

  Department Of Agriculture Kyoto University

• Yoshitake Takao

  Harmful Algal Bloom Division National Research Institute Of Fisheries And Environment Of Inland Sea

• Tomaru Yuji

  National Res. Inst. Of Fisheries And Environment Of Inland Sea Fisheries Res. Agency

• Tomaru Yuji

  Harmful Algal Bloom Division National Research Institute Of Fisheries And Environment Of Inland Sea

• Yoshida Takashi

  Dept. Mar. Biosci. Fukui Pref. Univ.

• Hiroishi Shingo

  Department of Bioscience, Fukui Prefectural University

• TAKASHIMA Yukari

  Department of Biomedical Engineering, Osaka Electro-Communication University

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