Isolation and characterization of a novel virus infecting Teleaulax amphioxeia (Cryptophyceae)

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概要

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• Teleaulax amphioxeia (Conrad) Hill is a marine free-living cryptophyte. Here, we report the basic characteristics of the cryptophyte-infecting virus “TampV (Teleaulax amphioxeia virus)”, the host of which is T. amphioxeia, as the first such virus to be successfully cultured. TampV strain 301 (TampV301) is a polyhedral large virus (ca. 203 nm in diameter), propagating in its hosts cytoplasm. Because of the virion size, thin-section view and propagation characteristics, TampV301 was assumed to harbor a large double-stranded (ds) DNA genome; i.e., TampV is most likely one of the “nucleo-cytoplasmic large DNA viruses (NCLDVs)” belonging to the family Phycodnaviridae. Its infectivity was ‘strain-specific’ rather than ‘species-specific’ as is the case in other algal viruses. The burst size and latent period were roughly estimated to be 430–530 infectious units cell−1 and <24 h, respectively. Considering the uniqueness of cryptophytes evolutionary position and the hosts unique role within the complicated food chain involving kleptoplastid acquisition (composed of T. amphioxeia, the ciliate Myrionecta rubra and a dinoflagellate Dinophysis species), TampV is of much interest from the viewpoints of both eukaryotic host-virus coevolution and marine microbial ecology.

• 日本プランクトン学会、日本ベントス学会の論文
• 2009-08-01

著者

• NAGASAKI KEIZO

  National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency

• Takao Yoshitake

  Department Of Agriculture Kyoto University

• NAGASAKI KEIZO

  Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea

• KIM JIN-JOO

  Department of Fisheries Biology, Pukyong National University

• TOMARU YUJI

  Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea

• TAKAO YOSHITAKE

  Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea

• NAGAI SATOSHI

  Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea

• Nagai Satoshi

  Harmful Algal Bloom Div. National Res. Inst. Of Fisheries And Environment Of Inland Sea Fisheries Re

• Nagasaki Keizo

  National Res. Inst. Of Fisheries And Environment Of Inland Sea Fisheries Res. Agency

• Nagasaki Keizo

  Harmful Algal Bloom Division National Research Institute Of Fisheries And Environment Of Inland Sea

• Yoshitake Takao

  Harmful Algal Bloom Division National Research Institute Of Fisheries And Environment Of Inland Sea

• Kim Jin-joo

  Department Of Fisheries Biology Pukyong National University

• Tomaru Yuji

  National Res. Inst. Of Fisheries And Environment Of Inland Sea Fisheries Res. Agency

• Nagai Satoshi

  Harmful Algal Bloom Division National Research Institute Of Fisheries And Environment Of Inland Sea

• Tomaru Yuji

  Harmful Algal Bloom Division National Research Institute Of Fisheries And Environment Of Inland Sea

• Nagai Satoshi

  Harmful Algal Bloom Division National Research Institute Of Fisheries And Environment Of Inland Sea

• Yoshida Takashi

  Dept. Mar. Biosci. Fukui Pref. Univ.

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