Development of compound microsatellite markers in the toxic dinoflagellate Alexandrium catenella (Dinophyceae)

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概要

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• In this study, we identified 9 polymorphic compound microsatellite markers in the toxic dinoflagellate Alexandrium catenella, which was isolated from Thau lagoon (France, Mediterranean Sea), using the compound microsatellite marker technique. These new microsatellites were characterized by screening DNA templates from 43 A. catenella clonal strains, which were collected from a seawater sample from Inokushi Bay (Oita Prefecture, Japan). These loci provide one class of highly variable genetic marker: the number of alleles ranged from 3 to 8, and the estimate of gene diversity varied between 0.285 and 0.762. We also analyzed the same 43 DNA samples using microsatellite markers previously identified for A. catenella, comparing the PCR amplification success, the number of alleles and gene diversity. These three parameters were not significantly different, showing that the compound microsatellite markers have the same potential to reveal A. catenella genetic structure. This simple and efficient method reduces the costs for developing SSR markers and for analyzing the genetic structure of populations, therefore, suggesting the effectiveness of applying this method to other species.

• 日本プランクトン学会、日本ベントス学会の論文
• 2007-08-01

著者

• NAGAI SATOSHI

  Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea

• Yasuda Nina

  Graduate School Of Information Science And Engineering Tokyo Institute Of Technology

• Nagai Satoshi

  Harmful Algal Bloom Div. National Res. Inst. Of Fisheries And Environment Of Inland Sea Fisheries Re

• ITAKURA Shigeru

  Harmful Phytoplankton Section, Harmful Algal Bloom Division, National Research Institute of Fisherie

• SEKINO Masashi

  Tohoku National Fisheries Research Institute

• Sekino Masashi

  Tohoku Natinal Fisheries Res. Inst. Miyagi Jpn

• NISHITANI GOH

  Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea

• MASSERET ESTELLE

  University Montpellier 2, Laboratoire Ecosystemes Lagunaires (UMR-CNRS 5119, ECOLAG)

• LIAN CHUNLIAN

  Asian Natural Environmental Science Center, the University of Tokyo

• YAMAGUCHI SANAE

  Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea

• GRZEBYK DANIEL

  University Montpellier 2, Laboratoire Ecosystemes Lagunaires (UMR-CNRS 5119, ECOLAG)

• BERREBI PATRICK

  University Montpellier 2, Institut des Sciences de l'Evolution (UMR-CNRS 5554)

• Itakura Shigeru

  Harmful Algal Bloom Division National Research Institute Of Fisheries And Environment Of Inland Sea

• Lian Chunlian

  Asian Natural Environmental Science Center The University Of Tokyo

• Nishitani Goh

  National Res. Inst. Fisheries And Environment Of Inland Sea Hiroshima Jpn

• Nishitani Goh

  Harmful Algal Bloom Division National Research Institute Of Fisheries And Environment Of Inland Sea

• Grzebyk Daniel

  University Montpellier 2 Laboratoire Ecosystemes Lagunaires (umr-cnrs 5119 Ecolag)

• Nagai Satoshi

  Harmful Algal Bloom Division National Research Institute Of Fisheries And Environment Of Inland Sea

• Berrebi Patrick

  University Montpellier 2 Institut Des Sciences De L'evolution (umr-cnrs 5554)

• Masseret Estelle

  University Montpellier 2 Laboratoire Ecosystemes Lagunaires (umr-cnrs 5119 Ecolag)

• Yamaguchi Sanae

  Harmful Algal Bloom Division National Research Institute Of Fisheries And Environment Of Inland Sea

関連論文

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• The seasonal occurrence of Alexandrium tamarense (Dinophyceae) vegetative cells in Hiroshima Bay, Japan
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• Genetic Variability and Relationships of Japanese Native Chickens Assessed by Means of Microsatellite Profiling Approach-Focusing on the Oh-Shamo (Japanese Large Game) and Its Related Breeds
• Development of a quantification assay for the cysts of the toxic dinoflagellate Alexandrium tamarense using real-time polymerase chain reaction
• Development of compound microsatellite markers in the toxic dinoflagellate Alexandrium catenella (Dinophyceae)
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