Immobilization of Paracoccus denitrificans Cells with Polyelectrolyte Complex and Denitrifying Activity of the Immobilized Cells
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概要
- 論文の詳細を見る
Whole cells from Paracoccus denitrificans IFO 12442 were immobilized with a polyelectrolyte complex composed of potassium poly(vinyl alcohol)sulfate(KPVS)and poly(diallyldimethylammonium chloride)(PDDA)by the following procedures : An excess of PDDA was first mixed with a cell suspension to aggregate cells, then KPVS was added to form a complex with excess PDDA and to entrap the aggregated cells. Electron microscopic analysis showed that the aggregated cells were entrapped or surrounded by an amorphous complex support. The rate of nitrate reduction or carbon consumption by the immobilized cells was almost the same as that by the free cells, as determined by anaerobic incubation using a non-growth medium contraining KNO_3 as a substrate and potassium aspartate as a carbon source. The immobilized cells exhibited activity at pH 4,at which the free cells lost their activity. The initial activity of the immobilized cells remained stable for at least one month in a phosphate buffer with gentle stirring.
- 社団法人日本生物工学会の論文
- 1986-12-25
著者
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NAKAMURA Isei
Institute of Applied Biochemistry, University of Tsukuba
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Nakamura I
Univ. Tsukuba Tsukuba‐shi Jpn
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Nakamura Isei
Institute Of Appried Biochemistry University Of Tsukuba
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Nakamura Isei
Department Of Fool And Nutrition Sagami Woman's University
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Nakamura Isei
Institute Of Applied Biochemistry The University Of Tsukuba
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KOKUFUTA ETSUO
Institute of Applied Biochemistry, University of Tsukuba
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Kokufuta E
Univ. Tsukuba Ibaraki Jpn
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Kokufuta Etsuo
Institute Of Applied Biochemistry University Of Tsukuba
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SHIMOHASHI MASATO
Institute of Applied Biochemistry, University of Tsukuba
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Shimohashi Masato
Institute Of Applied Biochemistry University Of Tsukuba
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