Coimmobilization of Nitrosomonas europaea and Paracoccus denitrificans Cells Using Polyelectrolyte Complex-Stabilized Calcium Alginate Gel
スポンサーリンク
概要
- 論文の詳細を見る
Calcium alginate gel (CAG) that withstands phosphate ions in the medium was prepared by reinforcing a network structure of the gel with a polyelectrolyte complex (PEC) consisting of potassium poly(vinyl alcohol) sulfate and trimethylammonium glycol chitosan iodide. The PEC-stabilized CAG beads were used as a supporting matrix for the coimmobilization of Nitrosomonas europaea ATCC 25978 cells and Paracoccus denitrificans IFO 12442 cells. The coimmobilized cells were aerobically cultured on a medium containing 3 mM of phosphate ions, using (NH_4)_2SO_4 as a substrate and ethanol as a carbon source. Ammonia was consumed without forming nitrite, indicating the concurrence of nitrification and denitrification in the same system. No breakage of the gel beads was observed during the cultivation. Repeated aerobic cultivations using a column packed with beads of coimmobilized cells had stable initial activity for at least one month.
- 社団法人日本生物工学会の論文
- 1987-12-25
著者
-
NAKAMURA Isei
Institute of Applied Biochemistry, University of Tsukuba
-
Nakamura Isei
Institute Of Applied Biochemistry The University Of Tsukuba
-
KOKUFUTA ETSUO
Institute of Applied Biochemistry, University of Tsukuba
-
Kokufuta Etsuo
Institute Of Applied Biochemistry University Of Tsukuba
-
YUKISHIGE MASAYA
Institute of Applied Biochemistry, University of Tsukuba
-
Yukishige Masaya
Institute Of Applied Biochemistry University Of Tsukuba
関連論文
- Decolorization of Molasses Waste Water by a Thermophilic Strain, Aspergillus fumigatus G-2-6(Microbiology & Fermentation Industry)
- Recovery of ^Cs by a Bioaccumulation System Using Rhodococcus erythropolis CS98
- Trichloroethylene Degradation by Cells of a Methane-Utilizing Bacterium, Methylocystis sp. M, Immobilized in Calcium Alginate
- Localization of a Biopolymer Produced by Rhodococcus erythropolis Grown on n-Pentadecane(Biological Chemistry)
- A Protein Bioflocculant Produced by Rhodococcus erythropolis(Biological Chemistry)
- Continuous Decolorization of Molasses Waste Water Using Immobilized Lactobacillus hilgardii Cells(Microbiology & Fermentation Industry)
- Screening of Anaerobic Bacteria with the Ability to Decolorize Molasses Melanoidin(Microbiology & Fermentation Industry)
- Selective Determination of Selenite and Selenate in Wastewater by Graphite Furnace AAS after Iron(III) Hydroxide Coprecipitation and Reductive Coprecipitation on Palladium Collector Using Hydrazinium Sulfate
- Coimmobilization of Nitrosomonas europaea and Paracoccus denitrificans Cells Using Polyelectrolyte Complex-Stabilized Calcium Alginate Gel
- Immobilization of Trichloroethylene-Degrading Bacterium, Methylocystis sp. Strain M in Different Matrices
- Continuous Column Denitrfication Using Polyelectrolyte Complex-Entrapped Paracoccus denitrificans Cells
- Immobilization of Paracoccus denitrificans Cells with Polyelectrolyte Complex and Denitrifying Activity of the Immobilized Cells
- New intelligent polymer gels: a self-oscillating gel with pacemaking and actuating functions
- Separation and Preliminary Characterization of Acidic Polysaccharides Produced by Enterobacter sp.
- Selective Determination of Selenium(IV) and Selenium(VI) in Waste Water by Graphite Furnace AAS after Reductive Coprecipitation on Tellurium Collector by Ascorbic Acid, Tin(II) Chloride and Hydrazinium Sulfate
- Factors Affecting the Production of the Open-ring Acid of Spiculisporic Acid by Penicillium spiculisporum
- Protocatechuate 3, 4-Dioxygenase from Nocardia erythropolis