The Incorporation and Accumulation of Supplemented Fatty Acids in Mucor hiemalis HA-30

概要

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Accumulation of free unsaturated fatty acids, added individually to the medium, into cellular triacylglycerol (TG) were examined using a fungus, Mucor hiemalis HA-30,which could produce the eicosapentaenoic acid (20 : 5n-3)-enriched TG. Linoleic (18 : 2n-6), γ-linolenic (18 : 3n-6), arachidonic acids (20 : 4n-6), and 20 : 5n-3 were favored for accumulation in TG and each fatty acid accumulated in TG at about 80% purity. However, docosahexaenoic acid (22 : 6n-3) was not efficiently accumulated in TG and its content was about 60%. Incorporation and accumulation of various fatty acids added to the cultivation medium were investigated using a model fatty acids mixture. Generally, unsaturated fatty acids were efficiently incorporated into cells more than saturated fatty acids. Fatty acids with odd-numbered double bonds such as 18 : 3n-3,18 : 3n-6,and 20 : 5n-3 showed the higher incorporation efficiency compared with those with even-numbered double bonds such as 18 : 2n-6,20 : 4n-6,and 22 : 6n-3. On the other hand, saturated and monounsaturated fatty acids of 16 : 0,18 : 0,and 18 : 1 were preferentially accumulated in microbial cells over other fatty acids.
社団法人日本農芸化学会の論文
1999-06-23