Binding Properties of Bacillus thuringiensis Cry1C δ-Endotoxin to the Midgut Epithelial Membranes of Culex pipiens(Biochemistry & Molecular Biology)
スポンサーリンク
概要
- 論文の詳細を見る
The Cry1C δ-endotoxin from Bacillus thuringiensis is toxic to both lepidopteran and dipteran insect larvae. To analyze the dipteran-specific insecticidal mechanisms, we investigated the properties of Cry1C binding to the epithelial cell membrane of the larval midgut from the mosquito Culex pipiens in comparison with dipteran-specific Cry4A. Immunohistochemical staining of the larval midgut sections from Culex pipiens showed that Cry1C and Cry4A bound to the microvilli of the epithelial cells. The Cry1C binding to brush border membrane vesicles from the mosquito larvae was specific and irreversible, and did not compete with Cry4A. By ligand blotting analyses, we detected several Cry1C-binding proteins, the Cry1C binding to which did compete with excess unlabeled Cry4A. These results suggested that Cry1C and Cry4A recognized the same binding site(s) on the epithelial cell surface but that their interaction with the target membrane differed.
- 社団法人日本農芸化学会の論文
- 2003-01-23
著者
-
SAKAI Hiroshi
Department of Applied Mathematics and Physics, Graduate School of Informatics, Kyoto University
-
Sakai H
Okayama Univ. Okayama Jpn
-
Sakai H
Department Of Bioscience And Biotechnology Okayama University
-
Sakai H
Department Of Pharmacology Nagasaki University Shool Of Dentistry
-
Kamauchi Shinya
Department Of Biological Sciences Graduate School Of Science Osaka University
-
Kamauchi Shinya
Department Of Bioscience And Biotechnology Okayama University
-
YAMAGIWA Masashi
Department of Bioscience and Biotechnology, Okayama University
-
ESAKI Motoyuki
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
-
OTAKE Kanao
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
-
Yamagiwa M
Department Of Bioscience And Biotechnology Okayama University
-
Yamagiwa Masashi
Department Of Bioscience And Biotechnology Okayama University
-
Otake Kanao
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
-
Esaki Motoyuki
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
-
Sakai Hiroshi
Department Of Agricultural Chemistry The University Of Tokyo
関連論文
- Similarity, Clustering, and Scaling Analyses for the Foreign Exchange Market : Comprehensive Analysis on States of Market Participants with High-Frequency Financial Data(Financial and Consumer Markets,Econophysics-Physical Approach to Social and Economic
- Performance Studies of a Laser Wire Beam Profile Monitor
- Fluorescence Microscopic Demonstration of Cathepsin K Activity as the Major Lysosomal Cysteine Proteinase in Osteoclasts
- Structural Analysis of Repetitive DNA Sequences in the Goat Growth Hormone Gene Region(Biological Chemistry)
- Cloning and Sequencing of Goat Growth Hormone Gene(Biological Chemistry)
- Tissue- and Development-specific Expression of Goat Insulin-like Growth Factor-I (IGF-I) mRNAs
- Dynamic Aspects in the Expression of the Goat Insulin-like Growth Factor-I (IGF-I)Gene : Diversity in Transcription and Post-transcription
- Beneficial Effect of Perindopril on Cardiac Sympathetic Nerve Activity and Brain Natriuretic Peptide in Patients With Chronic Heart Failure : Comparison With Enalapril
- PJ-512 Impact of Oxidative Stress on Plasma Adiponectin in Patients with Heart Failure(Heart failure, clinical(18)(M),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- Comparison of the Effects of Efonidipine and Amlodipine on Aldosterone in Patients with Hypertension
- Binding of an Engineered 130-kDa Insecticidal Protein of Bacillus thuringiensis var.israelensis to Insect Cell Lines
- Importance of the Central Region of 130-kDa Insecticidal Proteins of Bacillus thuringiensis var. israelensis for Their Activity in Vivo and in Vitro
- Effects of Bacillus thuringiensis var.israelensis 20-kDa Protein on Production of the Bti 130-kDa Crystal Protein in Escherichia coli
- Expression of the Two 130-kDa Protein Genes of Bacillus thuringiensis var. israelensis in Bacillus subtilis(Biological Chemistry)
- Insecticidal Activity of a Peptide Containing the 30th to 695th Amino Acid Residues of the 130-kDa Protein of Bacillus thuringiensis var. israelensis(Biological Chemistry)
- Insecticidal Activity of the Products of Two 130-kDa Protein Genes(Biological Chemistry)
- Cloning and Nucleotide Sequences of the Two 130 kDa Insecticidal Protein Genes of Bacillus thuringiensis var. israelensis(Biological Chemistry)
- Amino Acid Residues in the Allosteric Site of L-Lactate Dehydrogenase from Bifidobacterium longum(Biological Chemistry)
- Changes in Expression of Osteocalcin,Matrix Gla Protein and Osteopontin mRNAs during Experimental Tooth Movement in Rats
- Antigenicity of pro-osteocalcin in hard tissue: the authenticity to visualize osteocalcin-producing cells
- PJ-509 Transcardiac Difference of Aldosterone in Patients with Heart Disease(Heart Failure, Clinical 13 (M) : PJ85)(Poster Session (Japanese))
- PJ-418 Relationship Between Transcardiac Increase of C-Reactive Protein and Transcardiac Increase of Interleukin-6 in Patients with Dilated Cardiomyopathy(Cardiomyopathy, Clinical 4 (M) : PJ70)(Poster Session (Japanese))
- PJ-224 The Activity and Expression of Myocardial NADPH Oxidase Are Increased in Heart Failure.(Heart Failure, Basic 2 (M) : PJ38)(Poster Session (Japanese))
- OJ-225 Relationship Between Heart-type Fatty Acid-binding Protein and Left Ventricular Remodeling in Patients with Dilated Cardiomyopathy(Cardiomyopathy, Clinical 1 (M) : OJ26)(Oral Presentation (Japanese))
- FRS-191 Comparison of BMP and N terminal proBNP in Patients with Heart Failure(Heart Failure : Basic and Clinical Viewpoint (M) : FRS22)(Featured Research Session (English))
- Genomic Structure and 5'-Flanking Sequences of Rat N-Acetylglucosaminyltransferase I Gene and Regulatory Role of Its Transcriptional Diversity(Biochemistry & Molecular Biology)
- Characterization of Rat N-Acetylglucosaminyltransferase I Expressed in Esherichia coli
- Cloning of a cDNA Encoding N-Acetylglucosaminyltransferase I from Rat Liver and Analysis of Its Expression in Rat Tissues
- Processing of Osteocalcin by Cysteine Proteinases Alters the Intrinsic Calcium Binding Capability
- PJ-634 Plasma High-Molecular Weight Adiponectin in Patients with Chronic Heart Failure(Heart failure, clinical-17, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-633 Possible Contribution of Oxidative Stress and BNP on the Elevation of Adiponectin in Patients with Chronic Heart Failure(Heart failure, clinical-17, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- OJ-023 Simvastatin Improves Cardiac Sympathetic Nerve Activity in Patients with Heart Failure(Heart failure, clinical-04, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- ^Fe Mossbaur Spectroscopic Study on the Dynamics of Ferrocene Enclathrated in Deoxycholic Acid
- X-Ray Diffraction and Mossbauer Spectroscopic Studies of FePS_3-Alkylamines Intercalation Compounds
- Isothermal Tetragonal to Monoclinic Phase Transition around Room Temperature in 2.0mol% Yittria-doped Zirconia Ceramics
- Reddish Escherichia coli Cells Caused by Overproduction of Bacillus stearothermophilus Uroporphyrinogen III Methylase : Cloning, Sequencing, and Expression of the Gene
- Molecular Cloning of the Genes for Pyruvate Kinase of Two Bacilli, Bacillus psychrophilus and Bacillus licheniformis, and Comparison of the Properties of the Enzymes Produced in Escherichia coli
- A Neopullulanase-type α-Amylase Gene from Thermoactinomyces vulgaris R-47
- Conductance and Its Variance of Disordered Wires with Symplectic Symmetry in the Metallic Regime(Condensed Matter : Electronic Structure, Electrical, Magnetic and Optical Properties)
- Cloning and Nucleotide Sequence of the Pullulanase Gene of Thermus thermophilus HB8 and Production of the Enzyme in Escherichia coli(Biochemistry & Molecular Biology)
- OJ-050 Comparative Effect of Metoprolol and Carvedilol on Myocardial Oxidative Stress in Patients with Chronic Heart Failure(Heart failure, clinical-2 (M) OJ9,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circ
- Slightly Renal Dysfunction Influences on the Plsama N terminal proBNP Level in Patient with Heart Failure(Heart Failure, Clinical 13 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- P-450 Site-directed Mutagenesis and Functional Analysis of Phloroglucinol O-Methyltransferase from Rosa chinensis
- Crystal Structure of Pyruvate Kinase from Geobacillus stearothermophilus
- Structurally and Functionally Conserved Domains in the Diverse Hydrophilic Carboxy-Terminal Halves of Various Yeast and Fungal Na^+/H~+ Antiporters(Nhalp)^1
- The Influence of Proprioceptive Impairment on Hand Function in Patients With Cervical Myelopathy
- A Conserved Domain in the Tail Region of the Saccharomyces cerevisiae Na^+/H^+ Antiporter (Nha1p) Plays Important Roles in Localization and Salinity-Resistant Cell-Growth
- Purification and Some Properties of a Glucoamylase from Clostridium sp. G0005(Biological Chemistry)
- Serum Level of Uric Acid, Partly Secreted From the Failing Heart, is a Prognostic Marker in Patients With Congestive Heart Failure
- Prediction of Mortality by High-Sensitivity C-Reactive Protein and Brain Natriuretic Peptide in Patients With Dilated Cardiomyopathy
- PJ-782 Plasma N-terminal proBNP Level is More Sensitive Marker of Myocardial Ischemia Than BNP(Neurohumoral factors-3 (H) PJ137,Poster Session (Japanese),Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation
- Monoclonal Antibodies Recognizing Surface Residues of the β Subunit of Escherichia coli F_1 ATPase: Functional Importance of the Epitope Residues^1
- Distribution of Transmission Eigenvalues in Disordered Wires with Symplectic Symmetry(Condensed matter: electronic structure and electrical, magnetic, and optical properties)
- Contribution of iron associated with high-molecular-weight substances to the maintenance of the SPAD value of young leaves of barley under iron-deficient conditions(Plant Nutrition)
- Radiofrequency Radiation at 40 kHz Induces Hepatic Injury in Long-Evans Cinnamon (LEC) Rats, an Animal Model for Human Wilson Disease
- Immunolocalization of Heat Shock Proteins in the Retina of Normal Monkey Eyes and Monkey Eyes with Laser-induced Glaucoma
- PJ-062 Carperitide and Renal Function in Patients with Chronic Heart Failure(Heart failure, clinical-19 (M) PJ11,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- OJ-162 Myocardial Ischemia Itself Increases the Secretion of N-terminal proBNP from the Heart Independent of Hemodynamic Overload(Chronic coronary heart disease/Remodeling-2 (IHD) OJ27,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Me
- OJ-108 Prognostic Value of Combination of Serum Uric Acid and Brain Natriuretic Peptide in Patients with Chronic Heart Failure(Heart failure, clinical-4 (M) OJ18,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese C
- OJ-107 Plasma Active Renin Concentration is an Independent Prognostic Predictor in Patients with Chronic Heart Failure(Heart failure, clinical-4 (M) OJ18,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulati
- Is Transcardiac Increase of plasma N-terminal proBNP Level Related to Coronary Artery Stenosis in Patients with Ischemic Heart Disease? (Neurohumoral Factors 4 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Plasma High-Sensitive C-Reactive Protein as an Independent Prognostic Predictor in Patients with Dilated Cardiomyopathy(Cardiomyopathy, Clinical 8 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Inhibition of Aldosterone and Endothelin-1 by Carperitide was Attenuated More Than One Week Infusion in Patients with Congestive Heart Failure(Heart Failure, Clinical 14 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- OE-184 Plasma C-Reactive Protein as an Independent Prognostic Predictor in Patients with Dilated Cardiomyopathy(Heart Failure, Clinical 2 (M) : OE23)(Oral Presentation (English))
- Mossbauer Spectra of FePS_3 and Its Intercalation Compounds
- A case of fiberglass dermatitis
- Binding Properties of Bacillus thuringiensis Cry1C δ-Endotoxin to the Midgut Epithelial Membranes of Culex pipiens(Biochemistry & Molecular Biology)
- Active Form of Dipteran-Specific Insecticidal Protein Cry11A Produced by Bacillus thuringiensis subsp. israelensis(Biochemistry & Molecular Biology)
- Binding Properties of Bacillus thuringiensis Cry4A Toxin to the Apical Microvilli of Larval Midgut of Culex pipiens(Biochemistry & Molecular Biology)
- Conversion of Neopullulanase-α-Amylase from Thermoactinomyces vulgaris R-47 into an Amylopullulanase-Type Enzyme^1
- Characteristics of Response of Acid Phosphatase Secreted by the Roots of Several Crops to Various Conditions in the Growth Media
- Eleven-Year Follow Up of Bilateral Revision Total Hip Arthroplasty : A Case Report
- Ocular Findings of Beals Syndrome
- Ciliochoroidal Effusion Induced by Topical Latanoprost in a Patient with Sturge-Weber Syndrome
- Noise and Transmission Efficiency under Deformation of Tooth Form of Nylon Gear
- Lumbar Axial Loading Device Alters Lumbar Sagittal Alignment Differently From Upright Standing Position : A Computed Tomography Study
- Functional Analysis of Two Processed Fragments of Bacillus thuringiensis Cry11A Toxin
- Random-Matrix Theory of Electron Transport in Disordered Wires with Symplectic Symmetry(Condensed Matter: Electronic Structure, Electrical, Magnetic and Optical Properties)
- Effect of Atorvastatin vs. Rosuvastatin on Cardiac Sympathetic Nerve Activity in Non-Diabetic Patients With Dilated Cardiomyopathy
- Possible Structure and Function of the Extra C-Terminal Sequence of Pyruvate Kinase from Bacillus stearothermophilus
- Low- or Medium-pressure UV Lamp Inactivation of Microcystis aeruginosa
- An Overview of a Tool for Extracting Rules from Databases with Incomplete Information
- Mini-incision total hip arthroplasty : a quantitative assessment of laboratory data and clinical outcomes
- Mutagenesis of the Active Site Lysine 221 of the Pyruvate Kinase from Bacillus stearothermophilus
- Dendritic Growth of Polyoxymethylene Crystal
- Role of p38 Mapk in Development of Acute Hepatic Injury in Long-Evans Cinnamon (LEC) Rats, an Animal Model of Human Wilson's Disease
- A Useful Technique for Cephalic and Axillary Vein Puncture during Pacemaker Lead Implantation: How to Insert a Pacemaker Lead Has Been Established?
- Cation distribution in a series of mixed ferrites MgxMn1.1-xSn0.1Fe1.8O4 by means of powder X-ray diffraction and 57Fe, 119Sn Moessbauer spectroscopy.