Identification of the Promoter Region and the Transcriptional Regulatory Sequence of the evgAS Operon of Escherichia coli

スポンサーリンク

概要

• 論文の詳細を見る

• The evgAS operon of Escherichia coli encodes the EvgA response regulator and the EvgS sensory kinase, which are members of one of the two-component signal transduction systems of Escherichia coli. In this study, we identified the evg promoter and the EvgA-responsive element. Primer extension analysis found two evg transcritional initiation sites, designated P1 (+1) and P2 (-10), and placed them 114 bp and 124 bp upstream of evgA, respectively. A gel retardation assay demonstrated that EvgA specifically bound to an inverted repeat located between -102 and -128 counting from P1. We also did a β-galactosidase induction experiment using a promoter-probing vector and found that EvgA-binding sequence was important to stimulate the evg promoter. These results suggest that the expression of evgAS is positively regulated by its own product, EvgA.

• 社団法人日本農芸化学会の論文
• 1998-02-23

著者

• YOSHIOKA Sachiko

  Department of Chemistry, Faculty of Science, Saitama University

• Utsumi Ryutaro

  Department of Agricultural Chemistry, Kinki University

• Yamazaki Katsuhide

  Department of Agricultural Chemistry, Kinki University

• Tanabe Hiroyuki

  Department of Agricultural Chemistry, Kinki University

• Yoshioka Sachiko

  Department Of Agricultural Chemistry Kinki University

• Utsumi Ryutaro

  Department Of Agricultural Chemistry Kinki University

• Utsumi Ryutaro

  Department Of Advanced Bioscience Kinki University

• YAMASAKI Katsuhide

  Department of Agricultural Chemistry, Kinki University

• KATOH Akinori

  Department of Agricultural Chemistry, Kinki University

• Katoh Akinori

  Department Of Agricultural Chemistry Kinki University

• Yamazaki Katsuhide

  Department Of Agricultural Chemistry Kinki University

• Tanabe Hiroyuki

  Department Of Agricultural Chemistry Kinki University

関連論文

• Novel Non-benzenoid Electron Acceptors:Syntheses and Electron Acceptability of Tetracyanoazulenequinodimethanes and Dicyanoazulenequinonemethides
• CHARACTERIZATION OF THE SIGNAL TRANSDUCTION VIA EVGS AND EVGA IN ESCHERICHIA COLI
• X-Ray Crystal Structure of the DNA-Binding Domain of Response Regulator WalR Essential to the Cell Viability of Staphylococcus aureus and Interaction with Target DNA
• Antibacterial Agents that Inhibit Histidine Protein Kinase YycG of Bacillus subtilis(Biochemistry & Molecular Biology)
• Chemistry of Zerumbone. 2. Regulation of Ring Bond Cleavage and Unique Antibacterial Activities of Zerumbone Derivatives(Organic Chemistry)
• Identification and Characterization of a Potent Antibacterial Agent, NH125 against Drug-resistant Bacteria
• Mutational Analysis of the fic Promoter Recognized by RpoS (σ^) in Escherichia coli
• Isolation and Molecular Characterization of the Locked-on Mutant of Mg^ Sensor PhoQ in Escherichia coli
• Ascochytatin, a Novel Bioactive Spirodioxynaphthalene Metabolite Produced by the Marine-derived Fungus, Ascochyta sp. NGB4
• Binding of the Protein from Thermus aquaticus ISLtaq1 to Its Inverted Repeat in Vitro
• Identification of the Promoter Region and the Transcriptional Regulatory Sequence of the evgAS Operon of Escherichia coli
• Molecular Interaction between Proteins Involved in EvgAS Signal Transduction of Escherichia coli
• Inhibition of Cariogenic Factors of Mutans Streptococci by Persimmon Tannin
• Oxidative Arylation Mediated by Naphthalene-1,8-diylbis(diphenylmethylium) : Synthetic Route to Triarylsulfonium Salts
• Transition-metal-catalyzed Electrophilic Activation of 1,1-Difluoro-1-alkenes : Oxindole Synthesis via Intramolecular Amination
• X-Ray Crystal Structure of the DNA-Binding Domain of Response Regulator WalR Essential to the Cell Viability of Staphylococcus aureus and Interaction with Target DNA
• Essential Role of Phosphorylation in Signal Transduction by a Chimeric Receptor Composed of the Chemoreceptor Tar and Osmosensor EnvZ(Biological Chemistry)
• Isolation and Characterization of Inhibitors of the Essential Histidine Kinase, YycG in Bacillus subtilis and Staphylococcus aureus
• Isolation and Characterization of the IS3-like Element from Thermus aquaticus
• Transcriptional regulation of the Na^+-NADH : quinone oxidoreductase gene, nqr, in Vibrio anguillarum, a fish pathogen, in the stationary phase
• Molecular Cloning and Characterization of the Fusaric Acid-resistance Gene from Pseudomonas cepacia(Biological Chemistry)
• Isolation and Characterization of the Heat-responsive Genes in Escherichia coli
• Transcription of emrKY is Regulated by the EvgA-EvgS Two-Component System in Escherichia coli K-12
• Bacterial Signal Transduction Systems for Environmental Adaptation and Their Inhibitors
• Molecular Mechanism of Transcriptional Cascade Initiated by the EvgS/EvgA System in Escherichia coli K-12
• Walkmycin B targets WalK (YycG), a histidine kinase essential for bacterial cell growth
• Novel antibacterial compounds specifically targeting the essential WalR response regulator
• Mechanism of Activation of PhoQ/PhoP Two-Component Signal Transduction by SafA, an Auxiliary Protein of PhoQ Histidine Kinase in Escherichia coli

もっと見る 閉じる

スポンサーリンク