A new simple method to introduce marker-free deletions in the Bacillus subtilis genome
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概要
- 論文の詳細を見る
A genetic tool to introduce marker-free deletions is essential for multiple manipulations of genomes. We report a simple and efficient method to create marker-free deletion mutants of Bacillus subtilis through transformation with recombinant PCR products, using the Escherichia coli mazF gene encoding an endoribonuclease that cleaves free mRNAs as a counter-selection tool. Our method will be applicable to any bacterium in which introduction of the mazF cassette into the genome by double crossover homologous recombination is possible.
- 日本遺伝学会の論文
- 2009-08-25
著者
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MORIMOTO Takuya
Graduate School of Science and Technology, Yamagata University
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Morimoto Takuya
Graduate School Of Information Science Nara Institute Of Science And Technology
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ARA Katsutoshi
Biological Science Laboratories, Kao Co. Ltd
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OGASAWARA Naotake
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Ozaki Katsuya
Biological Science Laboratories, Kao Corporation
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Ara Katsutoshi
Biological Science Laboratories Kao Corporation
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Ozaki Katsuya
Biological Science Laboratories Kao Corporation
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Ogasawara Naotake
Graduate School Of Information Sci. Nara Inst. Of Sci. And Technol.
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Ogasawara Naotake
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Ara Katsutoshi
Biological Sci. Laboratories Kao Corp.
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Ozaki Katsuya
Biological Sci. Laboratories Kao Corp.
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