Isolation and Characterization of a Novel Enzyme, Nα-Benzyloxycarbonyl Amino Acid Urethane Hydrolase, from Streptococcus faecalis R ATCC 8043
スポンサーリンク
概要
- 論文の詳細を見る
A novel enzyme, which was named Nα-benzyloxycarbonyl amino acid urethane hydrolase, was purified from a cell-free extract of Streptococcus faecalis R ATCC 8043, using Nα-benzyloxycarbonyl glycine as substrate. The enzyme was purified 1300-fold with an activity yield of 8%. The purified enzyme was homogeneous by disc electrophoresis. The molecular weight of the native enzyme is about 220, 000 by gel filtration, and a molecular weight of 32, 000 was determined for the reduced and denatured enzyme by gel electrophoresis in sodium dodecyl sulfate. The isoelectric point was 4.48. The enzyme was inhibited by p-chloromercuribenzoate. The presence of divalent cations (i.e., Co2+ or Zn2+) is essential for its activity.
- 社団法人 日本農芸化学会の論文
著者
-
Murao Sawao
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
-
Kawano Tatsu
Laboratory Of Microbial Products Osaka University Of Pharmaceutical Sciences
-
MATSUMURA Eiko
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
-
MATSUMURA Eiko
Department of Agricultural Chemistry, Faculty of Agriculture, University of Osaka Prefecture
-
KAWANO Tatsu
Laboratory of Biochemistry, Osaka College of Pharmacy
関連論文
- Acetoxydehydroaustin, a New Bioactive Compound, and Related Compound Neoaustin from Penicillium sp. MG-11
- Identification of the Microorganism Which Produces Epstein-Barr Virus Early Antigen-inducing Indole Alkaloids
- Streptomyces Pepsin Inhibitor-insensitive Carboxyl Proteinase from Ganoderma lucidum
- A CLN2-Related and Thermostable Serine-Carboxyl Proteinase, Kumamolysin: Cloning, Expression, and Identification of Catalytic Serine Residue
- Nucleotide Sequence of the Gene Encoding Pepstatin-insensitive Acid Protease B, Scytalidopepsin B, of Scytalidium lignicolum
- A Novel Laccase Inhibitor, N-Hydroxyglycine, Produced by Penicillium citrinum YH-31
- New Enzymatic Colorimetric Analyses to Evaluate the Activities of Carboxypeptidase A and B(Analytical Chemistry)
- A New Screening Method for Microorganisms Which Produce Carboxypeptidases
- New Enzymatic Colorimetric Reactions of Benzoic Acid Derivatives with ABTS [2, 2-Azino-di-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] in the Presence of Laccase
- Purification and Some Properties of Urethane Hydrolase II from Lactobacillus casei ε ATCC 7469