Isolation and Characterization of a Novel Enzyme, Nα-Benzyloxycarbonyl Amino Acid Urethane Hydrolase, from Streptococcus faecalis R ATCC 8043
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概要
- 論文の詳細を見る
A novel enzyme, which was named Nα-benzyloxycarbonyl amino acid urethane hydrolase, was purified from a cell-free extract of Streptococcus faecalis R ATCC 8043, using Nα-benzyloxycarbonyl glycine as substrate. The enzyme was purified 1300-fold with an activity yield of 8%. The purified enzyme was homogeneous by disc electrophoresis. The molecular weight of the native enzyme is about 220, 000 by gel filtration, and a molecular weight of 32, 000 was determined for the reduced and denatured enzyme by gel electrophoresis in sodium dodecyl sulfate. The isoelectric point was 4.48. The enzyme was inhibited by p-chloromercuribenzoate. The presence of divalent cations (i.e., Co2+ or Zn2+) is essential for its activity.
- 社団法人 日本農芸化学会の論文
著者
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Murao Sawao
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Kawano Tatsu
Laboratory Of Microbial Products Osaka University Of Pharmaceutical Sciences
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MATSUMURA Eiko
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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MATSUMURA Eiko
Department of Agricultural Chemistry, Faculty of Agriculture, University of Osaka Prefecture
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KAWANO Tatsu
Laboratory of Biochemistry, Osaka College of Pharmacy
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