Isolation and Identification of a Hog Pancreatic α-Amylase Inhibitor (Haim) Producing Streptomyces
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概要
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A screening test was carried out to obtain microbes which produce hog pancreatic α-amylase inhibitor and a new inhibitor was found in culture broth of an actinomycete, strain YM-25.This inhibitor was designated as Haim, an abbreviation for hog pancreatic α-amylase inhibitor from a microbe. The determined morphological and physiological properties of strain YM-25 led to the conclusion that the microorganism was Streptomyces griseosporeus. When the microorganism was aerobically cultured at 30°C in a jar fermentor containing the most suitable medium for growth which consisted of 5% glycerol, 0.5% polypepton, 0.2% meat extract, 0.1% yeast extract, 0.4% Na2HPO4- 12H2O, 0.1% KH2PO4, and 0.05% MgSO4 - 7H2O (pH 7.3), the highest activity of Haim was obtained on 23-26 hr cultivation. Haim had specific inhibitory activities against animal α-amylases but not against microbial and plant α-amylases.
- 社団法人 日本農芸化学会の論文
著者
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Arai Motoo
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Arai Motoo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Murao Sawao
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Goto Akira
Department Of Cardiovascular Surgery And Internal Medicine Ohtemae General Hospital
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OHYAMA Kunio
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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MATSUI Yoshihiro
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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