Expression of Aspergillus aculeatus No. F-50 Cellobiohydrolase I (cbhI) and β-glucosidase 1 (bgl1) genes by Saccharomyces cerevisiae

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概要

• 論文の詳細を見る

• A cellobiohydrolase I (cbhI) and a β-glucosidase 1 (bgl1) gene of Aspergillus aculeatus were expressed in Saccharomyces cerevisiae. The transformed cells secreted the enzymes efficiently in an active form. The recombinant CBHI gave two bands of different molecular mass (110 and 90 kDa) and the recombinant BGL1 gave one band (180 kDa) by SDS-PAGE. The recombinant CBHI and BGL1 had the same enzymatical properties as the native enzyme except for the specific activity toward cellulosic substrates. By the combination of three different types of cellulases, FI-CMCase, CBHI, and BGL1,we could hydrolyze Avicel up to 59% under our experimental conditions.

• 社団法人日本農芸化学会の論文
• 1998-08-23

著者

• KAWAGUCHI Takashi

  Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehring

• TAKADA Goro

  Department of Pediatrics, Akita University School of Medicine

• Takada G

  Rare Sugar Research Center Kagawa University

• Takada Goro

  Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University

• Arai Motoo

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture

• Kawaguchi Takashi

  Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect

• Kawaguchi Takashi

  Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University

• Kawaguchi Takashi

  College Of Agriculture University Of Osaka Prefecture

• Kawaguchi Takashi

  Department Of Drug Safety Research Eisai Co. Ltd.

• Arai M

  Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University

• Arai Motoo

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

• Kawaguchi T

  Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect

• Tajima Gou

  Department Of Pediatrics Hiroshima University Graduate School Of Biomedical Sciences

• SUMITANI Junichi

  Department of Applied Biological Chemistry, and Research Institute for Advanced Science and Technolo

• Takata Goro

  Rare Sugar Research Center Kagawa University

• Sumitani J

  Graduate School Of Life And Environmental Sciences Osaka Prefecture University

• Kawaguchi Takashi

  Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied

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