Biochemical Preparation of L-Ribose and L-Arabinose from Ribitol : A New Approach

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概要

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• L-Ribose and L-arabinose were prepared biochemically from ribitol via a two-step reaction, by which the complete oxidation of ribitol to L-ribulose (〜98%) was achieved by the reaction of washed cells of Acetobacter aceti IFO 3281. The produced L-ribulose was then used as a substrate for the production of L-ribose and L-arabinose. The isomerizatin of L-ribulose to L-ribose and L-arabinose was carried out using L-ribose isomerase (L-RI) of Acinetobacter sp.strain DL-28 and L-arabinose isomerase (L-AI) of Mycobacterium smegmatis, respectively. At equilibrium, the ratio of L-ribose : L-ribulose was 70 : 30 and that of L-arabinose : L-ribulose was 99 : 10. After a simple purification treatment, both pentoses could be crystallized without the use of column chromatography. The crystals were confirmed as L-ribose and L-arabinose by High-performance liquid chromatography (HPLC), Infrared (IR), Nuclear magnetic resonance (NMR) and optical rotation measurements.

• 社団法人日本生物工学会の論文
• 1999-10-25

著者

• IZUMORI KEN

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• TAKADA Goro

  Department of Pediatrics, Akita University School of Medicine

• Bhuiyan S

  Kagawa Univ. Kagawa Jpn

• Bhuiyan Shakhawat

  Department Of Biochemistry And Food Sciences Faculty Of Agriculture Kagawa University

• Izumori K

  Rare Sugar Research Center Kagawa University

• Izumori Ken

  Rare Sugar Research Center Faculty Of Agriculture Kagawa University

• Izumori Ken

  松谷化学工業 開発研究所

• Ahmed Zakaria

  Department Of Biochemistry And Food Sciences Faculty Of Agriculture Kagawa University

• Takada G

  Rare Sugar Research Center Kagawa University

• Takada Goro

  Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University

• SHIMONISHI TSUYOSHI

  Department of Biochemistry and Food Sciences, Faculty of Agriculture, Kagawa University

• UTAMURA MASARU

  Department of Biochemistry and Food Sciences, Faculty of Agriculture, Kagawa University

• Izumori Ken

  Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University

• Izumori Ken

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Izumori Ken

  Department Of Biochemistry And Food Science Faculty Of Agriculture And Rare Sugar Research Center Ka

• Bhuiyan Shakhawat

  Depatrment Of Biochemistry And Food Sciences Faculty Of Agriculture Kagawa University

• Utamura Masaru

  Department Of Biochemistry And Food Sciences Faculty Of Agriculture Kagawa University

• Tajima Gou

  Department Of Pediatrics Hiroshima University Graduate School Of Biomedical Sciences

• Takata Goro

  Rare Sugar Research Center Kagawa University

• Hossain Shakhawat

  Department Of Bioresourcre Science Faculty Of Agriculture Kagawa University

• Shimonishi T

  National Food Res. Inst. Ibaraki Jpn

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