Immobilization of L-Rhamnose Isomerase and Its Application in L-Mannose Production from L-Fructose
スポンサーリンク
概要
- 論文の詳細を見る
A constitutive L-rhamnose isomerase (L-RI) from Pseudomonas sp. strain LL172 was immobilized on various types and sizes of chitopearl beads. The highest activity yield (80%) observed was that of _L-RI immobilized on chitopearl beads BCW 2603. The pH range for optimal activity and stability of the immobilized enzyme (pH 5-11) was found to be broader than that for free enzyme. The temperature for optimal activity (60℃), and the thermal stability (stable at 60℃ for 10 min) were similar to that of the free enzyme. However, immobilized enzyme as well as free enzyme required manganese ions for maximum activity. At equilibrium, the production of L-mannose from L-fructose was determined to be 30%, and the time required to reach equilibrium was inversely proportional to the amount of immobilized enzyme added. Thus, L-mannose could be prepared on a large scale simply by increasing the initial amounts of the reaction components. Moreover, the immobilized enzyme retained about 90% of the initial activity even after five repeated uses in a batch reaction.
- 社団法人日本生物工学会の論文
- 1997-12-25
著者
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IZUMORI KEN
Department of Bioresource Science, Faculty of Agriculture, Kagawa University
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Bhuiyan S
Kagawa Univ. Kagawa Jpn
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Bhuiyan Shakhawat
Department Of Biochemistry And Food Sciences Faculty Of Agriculture Kagawa University
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Izumori K
Rare Sugar Research Center Kagawa University
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Izumori Ken
Rare Sugar Research Center Faculty Of Agriculture Kagawa University
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Izumori Ken
松谷化学工業 開発研究所
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ITAMI YOSHIYUKI
Department of Bioresource Science, Faculty of Agriculture, Kagawa University
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Izumori Ken
Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University
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Izumori Ken
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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Izumori Ken
Department Of Biochemistry And Food Science Faculty Of Agriculture And Rare Sugar Research Center Ka
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Itami Yoshiyuki
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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Bhuiyan Shakhawat
Depatrment Of Biochemistry And Food Sciences Faculty Of Agriculture Kagawa University
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Itami Y
Kagawa Univ. Kagawa Jpn
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