A Novel Enzymatic Approach to the Massproduction of L-Galactose from L-Sorbose(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

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概要

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• Wild-type strain of Pseudomonas cichorii ST-24 was unable to grow on D-psicose and inductively produced D-tagatose 3-epimerase (D-TE) with D-tagatose as an inducer. We have isolated a constitutive mutant, designated strain Ka75, which had acquired a new ability to grow on a mineral salts medium containing D-psicose as a sole carbon source. The D-psicose-metabolizing mutant synthesized a high level of D-TE. When grown on the culture medium supplemented with Mn^<2+>, the mutant strain produced around 250-fold higher activity than did the parent strain. Enzymatic properties of the constitutive enzyme were similar to those of the wild-type. Using the immobilized D-TE and recombinant L-rhamnose isomerase (L-RhI) from Escherichia coli strain JM109, a two-step enzymatic reaction was performed for massproduction of a rare aldo-hexose monosaccharide, L-galactose, from a common one, L-sorbose. In the first step, L-sorbose was epimerized to L-tagatose in a yield of 28%. The L-tagatose obtained was utilized as a starting material for L-galactose preparation by the immobilized L-RhI. At equilibrium, approximately 30% L-tagatose was isomerized to L-galactose. Finally, 7.5 g of L-galactose was obtained from 100 g of L-sorbose, viz an overall yield of 7.5%. The product obtained was purified and identified to be L-galactose by specific optical rotation and high performance liquid chromatography (HPLC) analysis, and was ultimately confirmed by ^<13>C nuclear magnetic resonance (^<13>C NMR) and IR spectra.

• 社団法人日本生物工学会の論文
• 2004-06-25

著者

• IZUMORI KEN

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• TAKADA Goro

  Department of Pediatrics, Akita University School of Medicine

• Izumori K

  Rare Sugar Research Center Kagawa University

• Izumori Ken

  Rare Sugar Research Center Faculty Of Agriculture Kagawa University

• Izumori Ken

  Dep. Of Bioresource Sci. Fac. Of Agriculture Kagawa Univ.

• Izumori Ken

  松谷化学工業 開発研究所

• Izumori Ken

  Department Of Biochemistry And Food Sciences Faculty Of Agriculture Kagawa University

• Leang Khim

  Department Of Biochemistry And Food Science Faculty Of Agriculture And Rare Sugar Research Center Ka

• Takada G

  Rare Sugar Research Center Kagawa University

• Takata Goro

  Rare Sugar Res. Center And Fac. Of Agriculture Kagawa Univeristy

• Takada Goro

  Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University

• Granstrorm Tom

  香川大希少糖セ

• Granstroem T

  Rare Sugar Research Center Kagawa University

• Morimoto K

  Faculty Of Agriculture Kagawa University

• Morimoto Kenji

  Department Of Mechanical Systems Engineering Tokyo University Of Agriculture And Technology

• Morimoto Kenji

  Rare Sugar Research Center Faculty Of Agriculture Kagawa University

• Izumori Ken

  Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University

• Izumori Ken

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Takada Goro

  秋田大学 医学部小児外科学

• Izumori Ken

  Department Of Biochemistry And Food Science Faculty Of Agriculture And Rare Sugar Research Center Ka

• Menavuvu Buetusiwa

  Department of Biochemistry and Food Science, Faculty of Agriculture and Rare Sugar Research Center,

• Granstrom Tom

  Department of Biochemistry and Food Science, Faculty of Agriculture and Rare Sugar Research Center,

• MAEKAWA KANAKO

  Department of Biochemistry and Food Science, Faculty of Agriculture and Rare Sugar Research Center,

• Menavuvu Buetusiwa

  Department Of Biochemistry And Food Science Faculty Of Agriculture And Rare Sugar Research Center Ka

• Granstrom Tom

  Department Of Biochemistry And Food Science Faculty Of Agriculture And Rare Sugar Research Center Ka

• Maekawa Kanako

  Department Of Biochemistry And Food Science Faculty Of Agriculture And Rare Sugar Research Center Ka

• Tajima Gou

  Department Of Pediatrics Hiroshima University Graduate School Of Biomedical Sciences

• Takata Goro

  Rare Sugar Research Center Kagawa University

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