Cloning and Sequencing of an Endoglucanase Gene from Scopulariopsis brevicaulis TOF-1212,and Its Expression in Saccharomyces cerevisiae

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概要

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• The egI gene, encoding a major endoglucanase (EGI) of Scopulariopsis brevicaulis TOF-1212,was cloned and sequenced. The egI gene consisted of 868bp with one intron and encoded a protein of 229 amino acids with a calculated molecular mass of 22,392 daltons. The EGI was assigned to a family 45 of glycosyl hydrolases and showed high similarity with other fungal endoglucanases, especially with those of Humicola grisea and Fusarium oxysporum, on the basis of hydrophobic cluster analysis. The egI gene was expressed under the promoter of the phosphoglycerate kinase gene (PGK) in Saccharomyces cerevisiae. The transformed cells were able to secrete the enzyme efficiently in an active form.

• 社団法人日本農芸化学会の論文
• 2000-06-23

著者

• KAWAGUCHI Takashi

  Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehring

• TAKADA Goro

  Department of Pediatrics, Akita University School of Medicine

• Takada G

  Rare Sugar Research Center Kagawa University

• Takada Goro

  Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University

• Arai Motoo

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture

• MORIYAMA Yasushi

  Fundamental Research Center, TOTO Ltd.

• Moriyama Yasushi

  Fundamental Research Center Toto Ltd.

• Kawaguchi Takashi

  Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect

• Kawaguchi Takashi

  Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture University

• Kawaguchi Takashi

  College Of Agriculture University Of Osaka Prefecture

• Kawaguchi Takashi

  Department Of Drug Safety Research Eisai Co. Ltd.

• Arai M

  Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University

• Arai Motoo

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

• Kawaguchi T

  Department Of Applied Life Sciences Graduate School Of Life And Environmental Sciences Osaka Prefect

• NAKATANI Fumiki

  Department of Applied Biological Chemistry, College of Agriculture, and Research Institute for Advan

• SUMITANI Jun-ichi

  Department of Applied Biological Chemistry, College of Agriculture, and Research Institute for Advan

• Tajima Gou

  Department Of Pediatrics Hiroshima University Graduate School Of Biomedical Sciences

• Takata Goro

  Rare Sugar Research Center Kagawa University

• Sumitani J

  Graduate School Of Life And Environmental Sciences Osaka Prefecture University

• Sumitani Jun-ichi

  Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied

• Kawaguchi Takashi

  Department Of Applied Biological Chemistry College Of Agriculture And Research Institute Of Applied

• Nakatani Fumiki

  Department Of Applied Biological Chemistry College Of Agriculture And Research Institute For Advance

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