Cloning and Overexpression of the Xylitol Dehydrogenase Gene from Bacillus pallidus and Its Application to L-Xylulose Production

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概要

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• The xylitol dehydrogenase gene (xdh) of Bacillus pallidus was cloned and overexpressed in Escherichia coli using pQE60 vector, for the first time. The open reading frame of 759 bp encoded a 253 amino acid protein with a calculated molecular mass of 27,333 Da. The recombinant xylitol dehydrogenase (XDH) was purified to homogeneity by three-step column chromatography, producing a single SDS–PAGE band of 28 kDa apparent molecular mass. The enzyme exhibited maximal activity at 55 °C in glycine-NaOH buffer pH 11.0, with 66% of initial enzyme activity retained after incubation at 40 °C for 1 h. In further application of the recombinant bacterium to L-xylulose production from xylitol (initial concentration 5%) using a resting cell reaction, 35% L-xylulose was produced within 24 h. This result indicates that this recombinant XDH is applicable in the large-scale production of L-xylulose.

• 社団法人 日本農芸化学会の論文
• 2010-09-23

著者

• Izumori K

  Rare Sugar Research Center Kagawa University

• Izumori Ken

  Rare Sugar Research Center Faculty Of Agriculture Kagawa University

• Izumori Ken

  Dep. Of Bioresource Sci. Fac. Of Agriculture Kagawa Univ.

• Izumori Ken

  松谷化学工業 開発研究所

• Izumori Ken

  Department Of Biochemistry And Food Sciences Faculty Of Agriculture Kagawa University

• Takada Goro

  Department Of Pediatrics Hiroshima University Graduate School Of Biomedical Sciences

• Takada G

  Rare Sugar Research Center Kagawa University

• Takata Goro

  Rare Sugar Res. Center And Fac. Of Agriculture Kagawa Univeristy

• Takada Goro

  Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University

• Morimoto K

  Faculty Of Agriculture Kagawa University

• Morimoto Kenji

  Rare Sugar Research Center Kagawa University

• Poonperm Wayoon

  Rare Sugar Research Center, Kagawa University

• Poonperm Wayoon

  Rare Sugar Research Center And Faculty Of Agriculture Kagawa Univeristy

• Izumori Ken

  Rare Sugar Res. Center Kagawa Univ.

• Izumori Ken

  Department Of Biochemistry And Food Science Faculty Of Agriculture Kanagawa University

• Takada Goro

  秋田大学 医学部小児外科学

• Tajima Gou

  Department Of Pediatrics Hiroshima University Graduate School Of Biomedical Sciences

• Poonperm Wayoon

  Rare Sugar Research Center Kagawa University

• Morimoto Kenji

  Rare Sugar Res. Center Kagawa Univ.

• Takata Goro

  Rare Sugar Research Center Kagawa University

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