Archaeal Aldehyde Dehydrogenase ST0064 from Sulfolobus tokodaii, a Paralog of Non-Phosphorylating Glyceraldehyde-3-phosphate Dehydrogenase, Is a Succinate Semialdehyde Dehydrogenase
スポンサーリンク
概要
- 論文の詳細を見る
Aldehyde dehydrogenase ST0064, the closest paralog of previously characterized allosteric non-phosphorylating glyceraldehyde-3-phosphate (GAP) dehydrogenase (GAPN, ST2477) from a thermoacidophilic archaeon, Sulfolobus tokodaii, was expressed heterologously and characterized in detail. ST0064 showed remarkable activity toward succinate semialdehyde (SSA) (Km of 0.0029 mM and kcat of 30.0 s−1) with no allosteric regulation. Activity toward GAP was lower (Km of 4.6 mM and kcat of 4.77 s−1), and previously predicted succinyl-CoA reductase activity was not detected, suggesting that the enzyme functions practically as succinate semialdehyde dehydrogenase (SSADH). Phylogenetic analysis indicated that archaeal SSADHs and GAPNs are closely related within the aldehyde dehydrogenase superfamily, suggesting that they are of the same origin.
著者
-
Fushinobu Shinya
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
-
Ito Fumiaki
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Setsunan University
-
Wakagi Takayoshi
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
-
ITO Fumiaki
Department of Biotechnology, The University of Tokyo
-
CHISHIKI Hidehiro
Department of Biotechnology, The University of Tokyo
関連論文
- The Role of Conserved Arginine Residue in Loop 4 of Glycoside Hydrolase Family 10 Xylanases
- Crystallographic and Mutational Analyses of Substrate Recognition of Endo-α-N-acetylgalactosaminidase from Bifidobacterium longum
- Transglycosylation Activity of Dictyoglomus thermophilum Amylase A
- A Series of Crystal Structures of a meta-Cleavage Product Hydrolase from Pseudomonas fluorescens IP01 (CumD) Complexed with Various Cleavage Products
- Purification, Characterization, and Steady-State Kinetics of a meta-Cleavage Compound Hydrolase from Pseudomonas fluorescens IP01
- N-Linked Oligosaccharides of Aspergillus awamori Feruloyl Esterase Are Important for Thermostability and Catalysis
- Involvement of a Glu71-Arg64 Couple in the Access Channel for NADH in Cytochrome P450nor
- 2-Oxoacid: Ferredoxin Oxidoreductase from the Thermoacidophilic Archaeon, Sulfolobus sp. Strain 7
- RECQ5/QE DNA Helicase Interacts with Retrotransposon mdg3 gag, an HIV Nucleocapsid-Related Protein
- Experiments on Frequency Error Compensation Using Synchronization Channel in OFDM Radio Access
- Cloning of the Gene for Inorganic Pyrophosphatase from a Thermoacidophilic Archaeon, Sulfolobus sp. Strain 7,and Overproduction of the Enzyme by Coexpression of tRNA for Arginine Rare Codon
- High-Speed Video Observation of Tympanic Membrane Rupture in Guinea Pigs(Bioengineering)
- PROTON-INDUCED MEMBRANE FUSION OF LIPOSOMES CONTAINING pH-SENSITIVE POLYMER
- Roles of the HSP70-Subunit in a Eukaryotic Multi-Site-Specific Endonuclease, Endo. SceI : Autophosphorylation and Heat Stability
- Epidermal growth factor receptor lacking C-terminal autophosphorylation sites retains signal transduction and high sensitivity to epidermal growth factor receptor tyrosine kinase inhibitor
- Analyses of Bifidobacterial Glycosidases Involved in the Metabolism of Oligosaccharides
- Increase of the Protease Activity of Aqualysin I, a Thermostable Serine Protease, by Replacing Asn219 near the Catalytic Residue Ser222
- グライコシンターゼ化による反転型加水分解酵素のグリコシド合成触媒への変換
- Hepatocyte Growth Factor and Transforming Growth Factor-β Stimulate both Cell Growth and Migration of Human Gastric Adenocarcinoma Cells^1
- Sodium-23 and Lanthanum-139 Nuclear Magnetic Resonance Studies of Cation Binding to Aqualysin I, a Thermostable Serine Protease(Analytical Chemistry)
- A Eukaryotic Copper-Containing Nitrite Reductase Derived from a NirK Homolog Gene of Aspergillus oryzae
- The Possible Involvement of Copper-Containing Nitrite Reductase (NirK) and Flavohemoglobin in Denitrification by the Fungus Cylindrocarpon tonkinense
- Streptomyces griseus Enhances Denitrification by Ralstonia pickettii K50, Which Is Possibly Mediated by Histidine Produced during Co-Culture
- Molecular Anatomy of the Alkaliphilic Xylanase from Bacillus halodurans C-125
- Unique Sugar Metabolic Pathways of Bifidobacteria
- Structural Explanation for the Acquisition of Glycosynthase Activity
- Reconstitution of the Na^+/H^+ Antiporter : A New Method for the Determination of H^+ Efflux from Na^+/H^+ Antiporter-Reconstituted Vesicles
- Oxygen-Carrying Cobaltporphyrin Complexed with Electroconductive Poly(3,4-azopyridylene)
- Mutational Analysis of Fungal Family 11 Xylanases on pH Optimum Determination
- Interactions between Glycoside Hydrolase Family 94 Cellobiose Phosphorylase and Glucosidase Inhibitors
- Crystal Structures of Glycoside Hydrolase Family 51 α-L-Arabinofuranosidase from Thermotoga maritima
- Pentalenic acid is a shunt metabolite in the biosynthesis of the pentalenolactone family of metabolites : hydroxylation of 1-deoxypentalenic acid mediated by CYP105D7 (SAV_7469) of Streptomyces avermitilis
- A molecular design that stabilizes active state in bacterial allosteric L-lactate dehydrogenases
- Survival of the Aerobic Denitrifier Pseudomonas stutzeri Strain TR2 during Co-Culture with Activated Sludge under Denitrifying Conditions
- Bioaugmentation of a wastewater bioreactor system with the nitrous oxide-reducing denitrifier Pseudomonas stutzeri strain TR2(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Archaeal Aldehyde Dehydrogenase ST0064 from Sulfolobus tokodaii, a Paralog of Non-Phosphorylating Glyceraldehyde-3-phosphate Dehydrogenase, Is a Succinate Semialdehyde Dehydrogenase
- Foreword Target Therapy for Cancer: Anti-cancer Drugs Targeting Growth-Factor Signaling Molecules
- Effectiveness of heat treatment to protect introduced denitrifying bacteria from eukaryotic predatory microorganisms in a pilot-scale bioreactor(ENVIRONMENTAL BIOTECHNOLOGY)
- Tumor necrosis factor-induced permeability increase of negatively charged phospholipid vesicles.
- Bioaugmentation of a wastewater bioreactor system with the nitrous oxide-reducing denitrifier Pseudomonas stutzeri strain TR2