Visualizing Nuclear Migration during Conidiophore Development in Aspergillus nidulans and Aspergillus oryzae : Multinucleation of Conidia Occurs through Direct Migration of Plural Nuclei from Phialides and Confers Greater Viability and Early Germination i
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概要
- 論文の詳細を見る
Nuclear migration is indispensable for normal growth, differentiation, and development, and has been studied in several fungi including Aspergillus nidulans and Neurospora crassa. To better characterize nuclear movement and its consequences during conidiophore development, conidiation, and conidial germination, we performed confocal microscopy and time-lapse imaging on A. nidulans and Aspergillus oryzae strains expressing the histone H2B-EGFP fusion protein. Active trafficking of nuclei from a vesicle to a phialide and subsequently into a conidium provided the mechanistic basis for the formation of multinucleate conidia in A. oryzae. In particular, the first direct visual evidence on multinucleate conidium formation by the migration of nuclei from a phialide into the conidium, rather than by mitotic division in a newly formed conidium, was obtained. Interestingly, a statistical analysis on conidial germination revealed that conidia with more nuclei germinated earlier than those with fewer nuclei. Moreover, multinucleation of conidia conferred greater viability and resistance to UV-irradiation and freeze-thaw treatment.
- 社団法人 日本農芸化学会の論文
- 2005-04-23
著者
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Kitamoto Katsuhiko
Department of Biotechnology, The University of Tokyo
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Nakajima Hiroo
京都府立医科大学 内分泌･乳腺外科学部門
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Nakajima Hiroo
Tsujigiwa Daigo Clinic 1-19 Daigo Shinmachi-uramachi Fushimi-ku Kyoto 601-1326 Jpn
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Kitamoto K
Univ. Tokyo Tokyo Jpn
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Kitamoto K
National Research Institute Of Brewing
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Kitamoto Katsuhiko
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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MARUYAMA Jun-ichi
Department of Biotechnology, The University of Tokyo
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JUVVADI Praveen
Department of Biotechnology, The University of Tokyo
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Maruyama J
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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JUVVADI Praveen
Dept. Biotech., Univ. Tokyo
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ISHI Kazutomo
Department of Biotechnology, The University of Tokyo
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NAKAJIMA Harushi
Department of Biotechnology, The University of Tokyo
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Ishi Kazutomo
Dept. Biotechnol., Univ. Tokyo
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Kitamoto Katsuhiko
Department Of Biotechnology University Of Tokyo
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Maruyama Jun-ichi
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Juvvadi Praveen
Dept. Biotech. Univ. Tokyo
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Kitamoto Katsuhiko
National Research Institute Of Brewing:(present Address)department Of Biotechnology The University O
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Nakajima H
Department Of Endocrine Breast Surgery Kyoto Prefectural University Of Medicine
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Ishi Kazutomo
Department Of Biotechnology The University Of Tokyo
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Kitamoto Katsuhiko
Department Of Applied Biological Chemistry And Department Of Applied Biological Engineering Graduate
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Juvvadi Praveen
Department Of Biotechnology The University Of Tokyo
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Maruyama Jun-ichi
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Nakajima Harushi
Department Of Agricultural Chemistry The University Of Tokyo
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Nakajima Hidenori
Exploratory Research Laboratories Fujisawa Pharmaceutical Co. Ltd
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Kimura K
Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokkaido University
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Maruyama Junichi
Department Of Biotechnology The University Of Tokyo
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