Expression of an 87-kD-β-1,3-Glucanase of Bacillus circulans IAM1165 in Saccharomyces cerevisiae by Low-temperature Incubation

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概要

• 論文の詳細を見る

• A DNA segment encoding a signal peptide from yeast invertase was fused in frame to bglH gene encoding 87-kD-β-1,3-glucanase from Bacillus circulans IAM1165 and was expressed in the yeast Saccharomyces cerevisiae under the control of the GAL1 gene promoter. Yeast cells containing this fused gene produced active β-1,3-glucanase in the medium after a long period of incubation at low temperature. The enzyme produced by yeast was heterogeneous in size, and larger than the enzyme produced by Escherichia coli.

• 社団法人日本農芸化学会の論文
• 1993-12-23

著者

• HORIKOSHI Koki

  Department of Bioengineering, The Tokyo Institute of Technology

• Nakajima Hiroo

  京都府立医科大学 内分泌･乳腺外科学部門

• Nakajima Hiroo

  Tsujigiwa Daigo Clinic 1-19 Daigo Shinmachi-uramachi Fushimi-ku Kyoto 601-1326 Jpn

• NAKAJIMA Harushi

  Department of Biotechnology, The University of Tokyo

• Yamamoto M

  Obihiro Univ. Agriculture And Veterinary Medicine Obihiro Jpn

• Aono R

  Tokyo Inst. Technol. Yokohama Jpn

• AONO Rikizo

  Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology

• YAMAMOTO Mami

  Department of Virology II, National Institute of Infectious Diseases

• Yamamoto Midori

  Research Center For Glycoscience Advanced Institute Of Industrial Science And Technology (aist)

• Nakajima H

  Department Of Endocrine Breast Surgery Kyoto Prefectural University Of Medicine

• Aono Rikizo

  Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology

• Yamamoto M

  Research Center For Glycoscience Advanced Institute Of Industrial Science And Technology (aist)

• Yamamoto Mami

  Department Of Biological Information Graduate School Of Bioscience And Biotechnology Tokyo Institute

• Noguchi Kasumi

  Department of Bioengineering, Tokyo Institute of Technology

• Nakajima Harushi

  Department Of Agricultural Chemistry The University Of Tokyo

• Noguchi K

  Graduate School Of Bioscience And Biotechnology Yokohama Jpn

• Nakajima Hidenori

  Exploratory Research Laboratories Fujisawa Pharmaceutical Co. Ltd

• Horikoshi Koki

  Department Of Applied Chemistry Faculty Of Engineering Toyo University

• Aono Rikizo

  Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Techanology

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