Deterioration of Tolerance to hydrophobic Organic Solvents in a Toluene-Tolerant Strain of Pseudomonas putida under the Conditions Lowering Aerobic Respiration
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概要
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The growth curve (increase in the number of viable cells) of a toluene-tolerant strain Pseudomonas putida Px51T was not reproducible in the presence of harmful organic solvents, such as p-xylene and toluene. The survival often fluctuated the during late exponential phase of growth. The repetitive growth was obtained by maintaining pO_2 20-40% (v/v) in the culture flask. However, even under these aerobic conditions, the cells starved for a carbon source were killed by exposure to harmful solvents. The tolerance to organic solvents was lowered greatly by treatment with a proton conductor, carbonyl cyanide m-chlorophenylhydrazone (CCCP), or an electron transport chain inhibitor, sodium azide. Px51T treated with CCCP lost tolerance to a wide variety of organic solvents with logP_<ow> of 2.6-4.2,which the organism usually tolerates. These results indicate that the solvent tolerance of Px51T depends upon on energy produced by aerobic respiration.
- 1999-08-23
著者
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Aono Rikizo
Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
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Aono Rikizo
Department Of Biological Information Graduate School Of Bioscience And Biotechnology Tokyo Institute
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Noguchi Kasumi
Department of Bioengineering, Tokyo Institute of Technology
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TSUKAGOSHI Norihiko
Department of Biological Information, Tokyo Institute of Technology
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Noguchi Kasumi
Department Of Biological Information Graduate School Of Bioscience And Biotechnology Tokyo Institute
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Tsukagoshi Norihiko
Department Of Biological Information Tokyo Institute Of Technology
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Aono Rikizo
Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Techanology
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