Cloning and Expression of a Gene for an 87-kDa β-1,3-Glucanase of Bacillus circulans IAM1165 in Escherichia coli K-12
スポンサーリンク
概要
- 論文の詳細を見る
Partially Sau3AI-digested fragments of chromosomal DNA from Bacillus circulans IAM1165,a high producer of β-1,3-glucanases able to lyse fungal cell walls, were inserted into a BamHI site of the plasmid vector pHSG399. A gene for the glucanase was cloned in Escherichia coli K-12 by the shotgun method. An 8-kb inserted DNA directed synthesis of an 87-kDa endo-β-1,3-D-glucanase in E. coli. The β-glucanase gene was in a 2.6-kb EcoRI-SmaI segment within the insert DNA. The enzyme activity was found mainly in the periplasmic fraction of E. coli carrying the gene.
- 社団法人日本農芸化学会の論文
- 1992-11-23
著者
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Sato Michiko
Oral Ecol. Health & Infect. Niigata Univ.
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Sato Masakazu
Department Of Cardiology Surugadai Nihon University Hospital
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Yamamoto M
Obihiro Univ. Agriculture And Veterinary Medicine Obihiro Jpn
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Aono R
Tokyo Inst. Technol. Yokohama Jpn
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AONO Rikizo
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology
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YAMAMOTO Mami
Department of Virology II, National Institute of Infectious Diseases
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Yamamoto Midori
Research Center For Glycoscience Advanced Institute Of Industrial Science And Technology (aist)
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Aono Rikizo
Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
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Nogi Yuichi
Superbugs Project Of Exploratory Research For Advanced Technology C
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Yamamoto M
Research Center For Glycoscience Advanced Institute Of Industrial Science And Technology (aist)
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Yamamoto Mami
Department Of Biological Information Graduate School Of Bioscience And Biotechnology Tokyo Institute
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Sato M
Mercian Corp. Fujisawa Jpn
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HORIKOSHI Koki
o Riken Institute
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Sato Masakazu
Department Of Biomedical Science And Engineering Faculty Of Health Sciences Hokkaido University
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Aono Rikizo
Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Techanology
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SATO Masakazu
Oral Ecol. Health & infect., Niigata Univ.
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