Purification and Characterization of Cell Wall-associated N-Acetylmuramyl-L-alanine Amidase from Alkaliphihc Bacillus lentus C-125
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概要
- 論文の詳細を見る
Cells of the facultative alkaliphile Bacillus sp. C-125 grown at neutral pH autolyze rapidly in alkaline buffers of pH 9-10. Alkaline autolytic activity has been found mainly in the cell wall fraction. A peptidoglycan lytic enzyme was extracted from the cell wall fraction suspended in 4M LiCl. The enzyme was identified as N-acetylmuramyl-L-alanine amidase, with a molecular mass of 58kDa. At low salinity, the enzyme formed an aggregate of high molecular mass. The peptidoglycan lytic reaction of this enzyme happened at pH 9.0-10.5 at 37℃. Optimum pH for the reaction was 9.7-10.0. The enzyme was most active at 60℃ when assayed at pH 9.0.
- 社団法人日本農芸化学会の論文
- 1996-07-23
著者
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HORIKOSHI Koki
Department of Bioengineering, The Tokyo Institute of Technology
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AONO Rikizo
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology
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Aono Rikizo
Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
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SANADA Takafumi
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology
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Horikoshi Koki
Department Of Applied Chemistry Faculty Of Engineering Toyo University
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Horikoshi Koki
Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
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Sanada Takafumi
Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
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Aono Rikizo
Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Techanology
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