Molecular Cloning, Nucleotide Sequence, and Expression of the Structural Gene for Alkaline Serine Protease from Alkaliphilic Bacillus sp.221
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概要
- 論文の詳細を見る
The gene encoding an alkaline serine protease from alkaliphilic Bacillus sp.221 was cloned in Escherichia coli and expressed in Bacillus subtilis. An open reading frame of 1,140 bases, identified as the protease gene was preceded by a putative Shine-Dalgarno sequence (AGGAGG) with a spacing of 7 bases. The deduced amino acid sequence had a pre-pro-peptide of 111 residues followed by the mature protease comprising 269 residues. The alkaline protease from alkaliphilic Bacillus sp.221 had higher homology to the protease from alkaliphilic bacilli (82.1% and 99.6%) than to those from neutrophilic bacilli (60.6-61.7%). Also Bacillus sp.221 protease and other protease from alkaliphilic bacilli shared common amino acid changes and 4 amino acid deletions that seemed to be related to characteristics of the enzyme of alkaliphilic bacilli when compared to the proteases from neutrophilic bacilli.
- 社団法人日本農芸化学会の論文
- 1992-09-23
著者
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NAKAMURA Satoshi
Department of Neurosurgery, Tokyo Women's Medical University
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HORIKOSHI Koki
Department of Bioengineering, The Tokyo Institute of Technology
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Yamamoto M
Obihiro Univ. Agriculture And Veterinary Medicine Obihiro Jpn
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Aono R
Tokyo Inst. Technol. Yokohama Jpn
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KOBAYASHI Masato
Department of Electronics, Kyushy University
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KOBAYASHI Tetsuo
Microbiology Laboratory, The Institute of Physical and Chemical Research (RIKEN)
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AONO Rikizo
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology
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YAMAMOTO Mami
Department of Virology II, National Institute of Infectious Diseases
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Yamamoto Midori
Research Center For Glycoscience Advanced Institute Of Industrial Science And Technology (aist)
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Nakamura S
Dep. Of Biotechnology The Univ. Of Tokyo
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Aono Rikizo
Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
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Yamamoto M
Research Center For Glycoscience Advanced Institute Of Industrial Science And Technology (aist)
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Yamamoto Mami
Department Of Biological Information Graduate School Of Bioscience And Biotechnology Tokyo Institute
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TAKAMI Hideto
Department of Bioengineering, Tokyo Institute of Technology
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Takami H
The Deep Star Group Japan Marine Science And Technology Center
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Nakamura Satoshi
Department Of Botany Graduate School Of Science Kyoto University
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Horikoshi Koki
Department Of Applied Chemistry Faculty Of Engineering Toyo University
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Kobayashi T
Microbiology Laboratory The Institute Of Physical And Chemical Research
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Kobayashi Tetsuo
Microbiology Laboratory The Institute Of Physical And Chemical Research (riken):department Of Applie
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Nakamura Satoshi
Department Of Bioengineering Tokyo Institute Of Technology
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Aono Rikizo
Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Techanology
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Kobayashi Masato
Department Of Bioengineering Tokyo Institute Of Technology
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Nakamura Satoshi
National Institute Of Information And Communications Technology
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Nakamura Satoshi
Department Of Acoustic And Speech Research Advance Telecommunications Research Institute International
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NAKAMURA SATOSHI
Department o Biophysics and Biochemistry, Faculty of Science, University of Tokyo
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