Molecular Cloning and Nucleotide Sequence of the groEL Gene from the Alkaliphilic Bacillus sp. Strain C-125 and Reactivation of Thermally Inactivated α-Glucosidase by Recombinant GroEL

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概要

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• The groEL gene of the alkaliphilic Bacillus sp. strain C-125 was cloned in Escherichia coli and sequenced. The groEL gene encoded a polypeptide of 544 amino acids and was preceded by the incomplete groES gene, lacking its 5'-end. The sequence of the derived amino acids was 87.5% identical to that of B. subtilis, 85.4% identical to that of B. stearothemophilus, and 60.9% identical to that of E. coli. The GroEL protein was expressed in E. coli. Purified GroEL protected yeast α-glucosidase from irreversible aggregation at a high temperature and the addition of Mg-ATP was essential for reactivation of the α-glucosidase. The addition of E. coli GroES increased recovery of the enzyme activity, indicating that C-125 GroEL could function in coordination with E. coli GroES.

• 社団法人日本農芸化学会の論文
• 1996-10-23

著者

• KUDO Toshiaki

  Department of Applied Microbiology, The Institute of Physical and Chemical Research

• Kudo T

  Inst. Physical And Chemical Res. Saitama Jpn

• Xu Yi

  Microbiology Laboratory, The Institute of Physical and Chemical Research (RIKEN)

• KOBAYASHI Tetsuo

  Microbiology Laboratory, The Institute of Physical and Chemical Research (RIKEN)

• Xu Yi

  Microbiology Laboratory The Institute Of Physical And Chemical Research (riken)

• Kudo Toshiaki

  Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University

• Kobayashi Tetsuo

  Microbiology Laboratory The Institute Of Physical And Chemical Research (riken):department Of Applie

関連論文

• The Effect of pH on Heat-resistance of Spores of Alkalophilic Bacillus No. 2b-2
• Effect of pH and Sodium Ion on Germination of Alkalophilic Bacillus Species
• Molecular Cloning and Nucleotide Sequence of the groEL Gene from the Alkaliphilic Bacillus sp. Strain C-125 and Reactivation of Thermally Inactivated α-Glucosidase by Recombinant GroEL
• Molecular Cloning, Nucleotide Sequence, and Expression of the Structural Gene for Alkaline Serine Protease from Alkaliphilic Bacillus sp.221

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