Improvement of Alkaliphily of Bacillus Alkaline Xylanase by Introducing Amino Acid Substitutions Both on Catalytic Cleft and Protein Surface
スポンサーリンク
概要
- 論文の詳細を見る
Xylanase J (XynJ) from alkaliphilic Bacillus sp. 41M-1 is an alkaline xylanase. The crystal structure has been solved with XynJ. Improvement of the alkaliphily of XynJ was attempted by amino acid substitutions. Reinforcing the characteristic salt bridge in the catalytic cleft and introducing excess Arg residues on the protein surface shifted the optimum pH of the wild-type enzyme from 8.5 to 9.5.
- 社団法人 日本農芸化学会の論文
- 2009-04-23
著者
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NAKAMURA Satoshi
Department of Neurosurgery, Tokyo Women's Medical University
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YATSUNAMI Rie
Department of Bioengineering, Tokyo Institute of Technology
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FUKUI Toshiaki
Department of Bioengineering, Tokyo Institute of Technology
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KUMASAKA Takashi
Department of Life Science, Tokyo Institute of Technology
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TANAKA Nobuo
Department of Life Science, Tokyo Institute of Technology
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UMEMOTO Hirohito
Department of Bioscience, School of Science, Kitasato University
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Tanaka Nobuo
Department Of Intercultural Studies Faculty Of Cultural Studies Kyoto Sangyo University
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Tanaka Nobuo
Dep. Of Life Sci. Tokyo Inst. Of Technol.
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Fukui Toshiaki
Dep. Of Bioengineering Tokyo Inst. Of Technol.
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Yatsunami Rie
Dep. Of Bioengineering Tokyo Inst. Of Technol.
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INAMI Mayuko
Department of Bioengineering, Tokyo Institute of Technology
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Kumasaka Takashi
Dep. Of Life Sci. Tokyo Inst. Of Technol.
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Inami Mayuko
Department Of Bioengineering Tokyo Institute Of Technology
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Umemoto Hirohito
Department Of Bioengineering Tokyo Institute Of Technology
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Nakamura Satoshi
Department Of Botany Graduate School Of Science Kyoto University
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Yatsunami Rie
Department Of Bioengineering Tokyo Institute Of Technology
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Kumasaka Takashi
Department Of Life Science Tokyo Institute Of Technology
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Fukui Toshiaki
Department Of Bioengineering Tokyo Institute Of Technology
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Tanaka Nobuo
Department Of Agro-environmental Sciences Graduate School Of Bioresource And Bioenvironmental Scienc
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Nakamura Satoshi
Department Of Bioengineering Tokyo Institute Of Technology
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Fukui Toshiaki
Department Of Bioengineering Graduate School Of Bioscience And Biotechnology Tokyo Institute Of Technology
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Nakamura Satoshi
Department Of Acoustic And Speech Research Advance Telecommunications Research Institute International
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