In-vitro assessment of fabric structural composite scaffold applicable to tissue regeneration around implants

概要

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Using tissue engineering techniques, we developed osteogenetic graft materials composed of autogenous mesenchymal stem cells (MSCs) harvested from bone marrow and platelet-rich plasma (PRP) prepared from the patient's blood. In order to improve predict-ability of implant and periodontal regenerative therapies, we devised a fabric structural composite scaffold that maintains space for the transplanted cells to regenerate tissue. For the purpose of conducting in vitro verification of the osteogenetic potential of the osteoblast-like cells injected into the woven fabric composite scaffold, these cells were incubated for an extended period of time on the scaffold and evaluations were conducted on the scaffold interface. The in vitro evaluations verified the capacity of human MSCs adhering to the scaffold to differentiate into osteoblast-like cells under the defined differentiation inducing conditions, leading to significant mineralization. These results verify that the woven fabric composite scaffold, infused with gelled material formed with cultured osteogenetic cells and PRP, can be used as an osteogenetic graft material in bone regenerative therapy around implants. These materials now await further evaluation by animal trials.