Evaluation of a new scavenging method for the isolation of cigarette smoke components for use in animal studies
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概要
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There are few reports concerning the effects of cigarette-smoke on orthodontic tooth movement. By quantitatively measuring the components present in the cigarette smoke, we hoped to be able investigate their effects on tooth movement. This study was done to evaluate a practical method of obtaining mainstream cigarette smoke. We used distilled water, physiological saline solution and ethanol as scavenging solvents. The mainstream smoke was allowed to flow into a gas-washing bottle. The solutions were isolated using a separation process and analyzed to determine the amounts of nicotine and tar present. Samples were analyzed using ^1H nuclear magnetic resonance (^1H NMR). The chemical 1,2-diphenylethane was added to the ethanol solutions for use during NMR measurements. Each of the solvents was scavenged. Ethanol, in particular, provided the greatest amount of cigarette smoke components by weight. Nicotine and tar, which have characteristic signals in the NMR spectra, were isolated for each solvent. The NMR showed the standard amount of nicotine in the ethanol solvent. From these results, we concluded that ethanol is the most suitable solvent for collecting mainstream cigarette-smoke. Based on these results, we can quantify the concentration of the solution administered to an animal as we like.
- 2013-04-00
著者
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Nishiura Aki
Department of Orthodontics, Osaka Dental University
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Nishiura Aki
Department Of Orthodontics Osaka Dental University
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Makita Yoshimasa
Department Of Organic And Polymeric Materials Tokyo Institute Of Technology
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Fujiwara Shin-ichi
Department Of Biological Regulation Faculty Of Medicine Tottori University
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Tsuda Susumu
Department Of Applied Chemistry Graduate School Of Engineering Osaka University
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Nagaie Maya
Graduate School Of Dentistry (orthodontics) Osaka Dental University
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Matsumoto Naoyuki
Department Of Biological Safety National Institute For Agro-environmental Sciences
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Nagaie Maya
Graduate School of Dentistry (Orthodontics), Osaka Dental University
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Makita Yoshimasa
Department of Chemistry, Osaka Dental University
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Matsumoto Naoyuki
Department and Faculty of Engineering, Graduate School, Chiba Institute of Technology
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