An Improved DNA Extraction Method Using Skim Milk from Soils That Strongly Adsorb DNA
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概要
- 論文の詳細を見る
DNA extraction has been difficult from some types of soil. Seven soil samples from agricultural fields and a forest, which were mainly volcanic ash soils, were used. Soil DNA could be extracted from only two of them using a commercially available kit exploiting bead-beating. When skim milk was added to the extraction buffer at 40 mg g^<-1> soil, DNA could be detected by electrophoresis from all the samples, indicating that the DNA from lysed cells was adsorbed by soil colloids. The addition of skim milk did not affect PCR-DGGE profiles. The improved method is applicable to the analysis of molecular communities in soils which strongly adsorb DNA.
- 日本微生物生態学会の論文
著者
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Matsumoto Naoyuki
Department Of Orthodontics Osaka Dental University
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Takada-hoshino Yuko
Department Of Biological Safety National Institute For Agro-environmental Sciences
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Matsumoto Naoyuki
Department Of Biological Safety National Institute For Agro-environmental Sciences
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Matsumoto Naoyuki
Department and Faculty of Engineering, Graduate School, Chiba Institute of Technology
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