Enhanced Sequence Coverage in Tryptic Fragment Analysis by Two-Dimensional HPLC/MS Using a Monolithic Silica Capillary Column

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概要

• 論文の詳細を見る

• The HPLC/MS system, in which a monolithic silica capillary column is directly connected to an electronspray-ionization mass spectrometer, showed superior performance at high mobile phase linear velocity. A two-dimensional (2D) HPLC/MS system was established, using an ion-exchange particle-packed capillary column at the first dimension and a monolithic silica capillary column at the second dimension. In an analysis of tryptic fragments from bovine serum albumin, an 81% sequence coverage, obtained by the 2D-HPLC/MS system, increased by 23% as compared to a 1D-HPLC/MS system. This 2D-HPLC/MS system using a monolithic silica capillary column should be useful for enhancing sequence coverage of tryptic fragments in proteomics.

• 社団法人 日本農芸化学会の論文
• 2007-09-23

著者

• Ueda Mitsuyoshi

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• SUGAHARA Takuya

  Faculty of Agriculture, Ehime University

• Akiyama Koichi

  Integrated Center For Sciences Tarumi Station Ehime Univ.

• TANAKA Nobuo

  Department of Life Science, Tokyo Institute of Technology

• Sugahara Takuya

  Faculty Of Agriculture Ehime University

• YAMAUCHI Satoshi

  Faculty of Agriculture, Ehime University

• MARUYAMA Masafumi

  Faculty of Agriculture, Ehime University

• KISHIDA Taro

  Faculty of Agriculture, Ehime University

• Nakato Tomofumi

  Faculty of Agriculture, Ehime University

• MIMA Joji

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• Ishizuka Norio

  Emaus Kyoto Inc.

• Ueda Mitsuyoshi

  Division Of Applied Life Science Graduate School Of Agriculture Kyoto University

• FURUNO Masahiro

  GL Sciences Inc.

• Tanaka Nobuo

  Dep. Of Life Sci. Tokyo Inst. Of Technol.

• MORISAKA Hironobu

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• HATA Koji

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• TANIGAWA Tetsuya

  Chemco Scientific Co., Ltd.

• Tanigawa Tetsuya

  Chemco Scientific Co. Ltd.

• TSUCHIYA Masahiro

  Faculty of Agriculture, Ehime University

• Kishida Taro

  Faculty Of Agriculture Ehime University

• Yamauchi Satoshi

  Faculty Of Agriculture Ehime University

• Akiyama Koichi

  Integrated Center For Sciences (incs) Ehime University

• Maruyama Masafumi

  Faculty Of Agriculture Ehime University

• Nakato Tomofumi

  Faculty Of Agriculture Ehime University

• Tsuchiya Masahiro

  Faculty Of Agriculture Ehime University

• Morisaka Hironobu

  Faculty of Agriculture, Ehime University

• Hata Koji

  Faculty of Agriculture, Ehime University

• Mima Joji

  Faculty of Agriculture, Ehime University

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