Convenient and Sensitive Evaluation of a Superoxide Anion-Generating Reagent Methyl Viologen by Escherichia coli Harboring a soxS' : : gfp Reporter Plasmid
スポンサーリンク
概要
- 論文の詳細を見る
We constructed a reporter system to detect a superoxide-generating methyl viologen using SoxRS of Escherichia coli and GFP of Aequorea victoria. E. coli carrying this plasmid exhibited strong fluorescence when grown in the presence of a superoxide-generating reagent methyl viologen. The fluorescence intensity observed in the stationary phase culture of the transformant increased in response to the methyl viologen concentration in a range of 0.01 μM to 10 μM.
- 2004-12-23
著者
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Aono R
Tokyo Inst. Technol. Yokohama Jpn
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AONO Rikizo
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology
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Aono Rikizo
Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
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Ohtsu Iwao
Department Of Biological Information Tokyo Institute Of Technology
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Ohtsu Iwao
Department Of Bioengineering Tokyo Institute Of Technology
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SHIBUYA Akihito
Department of Biological Information, Tokyo Institute of Technology
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TSUKAGOSHI Norihiko
Department of Biological Information, Tokyo Institute of Technology
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DOKYU Noriyuki
Department of Biological Information, Tokyo Institute of Technology
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Tsukagoshi N
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
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Dokyu Noriyuki
Department Of Biological Information Tokyo Institute Of Technology
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Tsukagoshi Norihiko
Department Of Biological Information Tokyo Institute Of Technology
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Aono Rikizo
Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Techanology
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Shibuya Akihito
Department Of Biological Information Tokyo Institute Of Technology
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