Cloning and Structural Analysis of bglM Gene Coding for the Fungal Cell Wall-lytic β-1,3-Glucan-hydrolase BglM of Bacillus circulans IAM1165(Microbiology & Fermentation Technology)
スポンサーリンク
概要
- 論文の詳細を見る
Bacillus circulans IAM1165 produces isoforms of β-1,3-glucan-hydrolases. Of these enzymes, the 42-kDa enzyme BglM degrades Aspergillus oryzae cell walls the most actively. A gene coding for a BglM precursor consisting of 411 amino acid residues was cloned. The 27 N-terminal amino acid sequence of the precursor is a signal peptide. The 141 C-terminal amino acid sequence showed a motif of carbohydrate-binding module family 13. This domain bound to pachyman, lichenan, and A. oryzae cell walls. The central domain showed a bacterial β-1,3-glucan-hydrolase motif belonging to glycosyl hydrolase family 16. By removal of the C-terminal domain in the IAM1165 culture, mature BglM was processed to several 27-kDa fragments that hydrolyze a soluble β-1,3-glucan.
- 社団法人日本農芸化学会の論文
- 2002-06-23
著者
-
Asano Takeshi
Department Of Gastroenterology Shizuoka General Hospital
-
Aono Rikizo
Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
-
Yamamoto Mami
Department Of Biological Information Graduate School Of Bioscience And Biotechnology Tokyo Institute
-
Asano T
Department Of Biological Information Graduate School Of Bioscience And Biotechnology Tokyo Institute
-
TAKI Jiro
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institu
-
Taki Jiro
Department Of Biological Information Graduate School Of Bioscience And Biotechnology Tokyo Institute
-
Aono Rikizo
Department Of Biological Information Graduate School Of Bioscience And Biotechnology Tokyo Institute
-
Aono Rikizo
Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Techanology
-
Asano Takeshi
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology
関連論文
- Cervical Epidural Arteriovenous Fistula With Radiculopathy Mimicking Cervical Spondylosis
- Cervical Perimedullary Arteriovenous Fistula in an Infant Presenting With Subarachnoid Hemorrhage
- Acute pancreatitis complicating typhoid fever in a 4-year-old girl
- Usefulness of DNA analysis of tuberculosis with ruptured lymphadenitis in a bacille Calmette-Guerin-vaccinated infant
- Cytological analysis of ascitic fluid from an adolescent male with intra-abdominal desmoplastic small round cell tumor
- ApoB C7623T polymorphism predicts risk for steroid-induced osteonecrosis of the femoral head after renal transplantation
- Genetic analysis of steroid-induced osteonecrosis of the femoral head
- PANCREATIC PLEURAL EFFUSION SUCCESSFULLY TREATED BY ENDOSCOPIC PANCREATIC DUCT DRAINAGE COMBINED WITH EXTRACORPOREAL SHOCK-WAVE LITHOTRIPSY : REPORT OF A CASE
- A Recurrent Case of Spontaneous Mediastinal Emphysema
- The Cell Surface Glycoprotein of Haloarcula japonica TR-1
- Self-assembly of sapovirus recombinant virus-like particles from polyprotein in mammalian cells
- Analysis of Organic Solvent Tolerance in Escherichia coli Using Gene Expression Profiles from DNA Microarrays
- Control with Organic Solvents of Efficiency of Persolvent Cholesterol Fermentation by Pseudomonas sp. Strain ST-200
- soxRS Gene Increased the Level of Organic Solvent Tolerance in Escherichia coli
- A Close Correlation between Improvement of Organic Solvent Tolerance Levels and Alteration of Resistance toward Low Levels of Multiple Antibiotics in Escherichia coli
- Convenient and Sensitive Evaluation of a Superoxide Anion-Generating Reagent Methyl Viologen by Escherichia coli Harboring a soxS' : : gfp Reporter Plasmid
- Transcriptional Analysis of the ostA/imp Gene Involved in Organic Solvent Sensitivity in Escherichia coli
- Deterioration of Tolerance to hydrophobic Organic Solvents in a Toluene-Tolerant Strain of Pseudomonas putida under the Conditions Lowering Aerobic Respiration
- Thermophilic Alkaline Xylanase from Newly Isolated Alkaliphilic and Thermophilic Bacillus sp. Strain TAR-1
- Degradation of Human Hair by a Thermostable Alkaline Protease from Alkaliphilic Bacillus sp. No.AH-101
- Molecular Cloning, Nucleotide Sequence, and Expression of the Structural Gene for Alkaline Serine Protease from Alkaliphilic Bacillus sp.221
- A Novel Gene Required for the Alkaliphily of the Facultative Alkaliphile Bacillus sp. Strain C-125
- Solid and cystic tumor of the pancreas in a 12-year-old boy
- Alkaline Growth pH-Dependent Increase of Respiratory and NADH-Oxidation Activities of the Facultatively Alkaliphilic Strain Bacillus lentus C-125
- Construction of a New Teichuronopeptide-defective Derivative from Alkaliphilic Bacillus sp. C-125 by Cell Fusion
- Expression of an 87-kD-β-1,3-Glucanase of Bacillus circulans IAM1165 in Saccharomyces cerevisiae by Low-temperature Incubation
- Effective Isolation and Identification of Toluene-tolerant Pseudomonas Strains
- Extracellular Production of Human Immunoglobulin ε-Chain/γ1-Chain Chimeric Fc Polypeptide by Escherichia coli
- Extracellular Production of Human Tumor Necrosis Factor-α by Escherichia coli Using a Chemically-synthesized Gene(Biological Chemistry)
- High Hydrostatic Pressure Treatment Impairs AcrAB-TolC Pump Resulting in Differential Loss of Deoxycholate Tolerance in Escherichia coli(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Cloning and Expression of a Gene for an 87-kDa β-1,3-Glucanase of Bacillus circulans IAM1165 in Escherichia coli K-12
- Risk factors for nontraumatic osteonecrosis of the femoral head after renal transplantation
- Minimum 10-year radiographic follow-up of a cementless acetabular component for primary total hip arthroplasty with a bulk autograft
- Effects of Organic Solvents on Growth of Escherichia coli K-12
- Mapping of Organic Solvent Tolerance Gene ostA in Escherichia coli K-12
- Regeneration of Protoplasts Prepared from Alkaliphilic Strains of Bacillus spp.
- Structure of the 87-kDa β-1,3-Glucanase Gene of Bacillus circulans IAM1165 and Properties of the Enzyme Accumulated in the Periplasm of Escherichia coli Carrying the Gene
- Isolation of Novel Toluene-Tolerant Strain of Pseudomonas aeruginosa
- Preparation of Organic Solvent-tolerant Mutants from Escherichia coli K-12(Microbiology & Fermentation Industry)
- Purification and Characterization of Cell Wall-associated N-Acetylmuramyl-L-alanine Amidase from Alkaliphihc Bacillus lentus C-125
- Effect of Magnetite on the Hematocrit Value in Exsanguinated Rats
- pH-Dependent Flagella Formation by Facultative Alkaliphilic Bacillus sp. C-125
- Changes in the duration of the urinary abnormality of purpura nephritis over the past 5 years
- Acute lymphoblastic leukemia Ehlers-Danlos syndrome
- Effects of Organic Solvents on Indigo Formation by Pseudomonas sp. strain ST-200 Grown with High Levels of Indole
- Cloning and Structural Analysis of bglM Gene Coding for the Fungal Cell Wall-lytic β-1,3-Glucan-hydrolase BglM of Bacillus circulans IAM1165(Microbiology & Fermentation Technology)
- Lithocholic Acid Side-chain Cleavage to Produce 17-Keto or 22-Aldehyde Steroids by Pseudomonas putida strain ST-491 Grown in the Presence of an Organic Solvent, Diphenyl Ether
- Isolation of Paenibacillus illinoisensis That Produces Cyclodextrin Glucanotransferase Resistant to Organic Solvents(Microbiology & Fermentation Technology)
- Stereospecific Oxidation of 3β-Hydroxysteroids by Persolvent Fermentation with Pseudomonas sp. ST-200
- Carotenes Produced by Alkaliphilic Yellow-pigmented Strains of Bacillus(Microbiology & Fermentation Industry)
- Decrease in Cytoplasmic pH-Homeostastatic Activity of the Alkaliphile Bacillus Lentus C-125 by a Cell Wall Defect(Biochemistry & Molecular Biology)
- Combination of extracorporeal membrane oxygenation and high-frequency oscillatory ventilation saved a child with severe ARDS after pulmonary resection
- Hyper-autolysis of the Facultative Alkaliphile Bacillus sp. C-125 Cells Grown at Neutral pH : Culture-pH Dependent Cross-linking of the Peptide Moieties of the Peptidoglycan
- Accumulation of Alkaliphilic Bacillus Penicillinase Cleaved within the Signal Sequence in Cytoplasm of Escherichia coli
- Hemophilic Pseudotumor of the Temporal Bone With Conductive Hearing Loss : Case Report
- Favorable Outcome After Radical Resection and Subsequent Local Irradiation of Malignant Peripheral Nerve Sheath Tumor in the Cervical Spine
- Hemophilic Pseudotumor of the Temporal Bone With Conductive Hearing Loss:—Case Report—
- Transformation From Asymptomatic to Symptomatic of Lower Cervical Spinal Dural Arteriovenous Fistula:—Case Report—