Stereospecific Oxidation of 3β-Hydroxysteroids by Persolvent Fermentation with Pseudomonas sp. ST-200
スポンサーリンク
概要
- 論文の詳細を見る
Pseudomonas sp. strain ST-200 isolated from a humus soil effectively oxidizes cholesterol dissolved in organic solvents but not that suspended in the growth medium. The organism does not assimilate cholesterol. This organism oxidized a variety of 5α- or 5-ene-sterols dissolved in organic solvent. First, the 3β-OH group was oxidized to a ketone group. The 3α-OH group was scarcely oxidized. Successively, C-6 position of 5-ene-steroids was hydroxylated, and a double bond of 5-ene-steroids was transferred from Δ^5 to Δ^4. Then, the 6-OH group was oxidized to a ketone group. Persolvent fermentation with ST-200 would provide an effective, convenient, and stereospecific method to oxidize the C-3 and C-6 positions of steroids.
- 社団法人日本農芸化学会の論文
- 1996-07-23
著者
-
Doukyu N
Tokyo Inst. Technol. Yokohama Jpn
-
Doukyu Noriyuki
Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
-
Aono Rikizo
Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
-
Aono Rikizo
Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Techanology
関連論文
- Time-Course Data Analysis of Gene Expression Profiles Reveals purR Regulon Concerns in Organic Solvent Tolerance in Escherichia coli(Microbial Physiology and Biotechnology)
- The Cell Surface Glycoprotein of Haloarcula japonica TR-1
- Analysis of Organic Solvent Tolerance in Escherichia coli Using Gene Expression Profiles from DNA Microarrays
- Control with Organic Solvents of Efficiency of Persolvent Cholesterol Fermentation by Pseudomonas sp. Strain ST-200
- soxRS Gene Increased the Level of Organic Solvent Tolerance in Escherichia coli
- A Close Correlation between Improvement of Organic Solvent Tolerance Levels and Alteration of Resistance toward Low Levels of Multiple Antibiotics in Escherichia coli
- Convenient and Sensitive Evaluation of a Superoxide Anion-Generating Reagent Methyl Viologen by Escherichia coli Harboring a soxS' : : gfp Reporter Plasmid
- Transcriptional Analysis of the ostA/imp Gene Involved in Organic Solvent Sensitivity in Escherichia coli
- Deterioration of Tolerance to hydrophobic Organic Solvents in a Toluene-Tolerant Strain of Pseudomonas putida under the Conditions Lowering Aerobic Respiration
- Thermophilic Alkaline Xylanase from Newly Isolated Alkaliphilic and Thermophilic Bacillus sp. Strain TAR-1
- Degradation of Human Hair by a Thermostable Alkaline Protease from Alkaliphilic Bacillus sp. No.AH-101
- Molecular Cloning, Nucleotide Sequence, and Expression of the Structural Gene for Alkaline Serine Protease from Alkaliphilic Bacillus sp.221
- A Novel Gene Required for the Alkaliphily of the Facultative Alkaliphile Bacillus sp. Strain C-125
- Alkaline Growth pH-Dependent Increase of Respiratory and NADH-Oxidation Activities of the Facultatively Alkaliphilic Strain Bacillus lentus C-125
- Construction of a New Teichuronopeptide-defective Derivative from Alkaliphilic Bacillus sp. C-125 by Cell Fusion
- Expression of an 87-kD-β-1,3-Glucanase of Bacillus circulans IAM1165 in Saccharomyces cerevisiae by Low-temperature Incubation
- Effective Isolation and Identification of Toluene-tolerant Pseudomonas Strains
- Extracellular Production of Human Immunoglobulin ε-Chain/γ1-Chain Chimeric Fc Polypeptide by Escherichia coli
- Extracellular Production of Human Tumor Necrosis Factor-α by Escherichia coli Using a Chemically-synthesized Gene(Biological Chemistry)
- High Hydrostatic Pressure Treatment Impairs AcrAB-TolC Pump Resulting in Differential Loss of Deoxycholate Tolerance in Escherichia coli(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Cloning and Expression of a Gene for an 87-kDa β-1,3-Glucanase of Bacillus circulans IAM1165 in Escherichia coli K-12
- Effects of Organic Solvents on Growth of Escherichia coli K-12
- Mapping of Organic Solvent Tolerance Gene ostA in Escherichia coli K-12
- Regeneration of Protoplasts Prepared from Alkaliphilic Strains of Bacillus spp.
- Structure of the 87-kDa β-1,3-Glucanase Gene of Bacillus circulans IAM1165 and Properties of the Enzyme Accumulated in the Periplasm of Escherichia coli Carrying the Gene
- Isolation of Novel Toluene-Tolerant Strain of Pseudomonas aeruginosa
- Preparation of Organic Solvent-tolerant Mutants from Escherichia coli K-12(Microbiology & Fermentation Industry)
- Purification and Characterization of Cell Wall-associated N-Acetylmuramyl-L-alanine Amidase from Alkaliphihc Bacillus lentus C-125
- Effect of Magnetite on the Hematocrit Value in Exsanguinated Rats
- pH-Dependent Flagella Formation by Facultative Alkaliphilic Bacillus sp. C-125
- Effects of Organic Solvents on Indigo Formation by Pseudomonas sp. strain ST-200 Grown with High Levels of Indole
- Cloning and Structural Analysis of bglM Gene Coding for the Fungal Cell Wall-lytic β-1,3-Glucan-hydrolase BglM of Bacillus circulans IAM1165(Microbiology & Fermentation Technology)
- Lithocholic Acid Side-chain Cleavage to Produce 17-Keto or 22-Aldehyde Steroids by Pseudomonas putida strain ST-491 Grown in the Presence of an Organic Solvent, Diphenyl Ether
- Isolation of Paenibacillus illinoisensis That Produces Cyclodextrin Glucanotransferase Resistant to Organic Solvents(Microbiology & Fermentation Technology)
- Stereospecific Oxidation of 3β-Hydroxysteroids by Persolvent Fermentation with Pseudomonas sp. ST-200
- Carotenes Produced by Alkaliphilic Yellow-pigmented Strains of Bacillus(Microbiology & Fermentation Industry)
- Decrease in Cytoplasmic pH-Homeostastatic Activity of the Alkaliphile Bacillus Lentus C-125 by a Cell Wall Defect(Biochemistry & Molecular Biology)
- Hyper-autolysis of the Facultative Alkaliphile Bacillus sp. C-125 Cells Grown at Neutral pH : Culture-pH Dependent Cross-linking of the Peptide Moieties of the Peptidoglycan
- Accumulation of Alkaliphilic Bacillus Penicillinase Cleaved within the Signal Sequence in Cytoplasm of Escherichia coli