Production and Product Quality Assessment of Human Hepatitis B Virus Pre-S2 Antigen in Submerged and Solid-State Cultures of Aspergillus oryzae
スポンサーリンク
概要
- 論文の詳細を見る
Pre-S2 is a diagnostically important antigen of human hepatitis B virus(HBV). In order to produce pre-S2 antigen in Aspergillus oryzae, the gene [pre-S2]_3, which encodes a tandemly triplicated repeat of pre-S2 polypeptides was fused with the partial glaA gene encoding glucoamylase lacking the starch-binding domain. In submerged culture, A.oryzae transformants carrying glaA-[pre-S2]_3 secreted a heterogeneously glycosylated form of the fusion protein that was partially degraded. Contrarily, utilization of a wheat bran solid-state culture system resulted in the secretion of a homogeneous glycosylated form of the whole fusion protein. This is the first report of a dissimilarity in glycosylated modification between submerged and solid-state culture conditions in heterologous protein production in A.oryzae.
- 社団法人日本生物工学会の論文
- 2000-07-25
著者
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Kitamoto Katsuhiko
Department of Biotechnology, The University of Tokyo
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Nakajima Hiroo
京都府立医科大学 内分泌･乳腺外科学部門
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Nakajima Hiroo
Tsujigiwa Daigo Clinic 1-19 Daigo Shinmachi-uramachi Fushimi-ku Kyoto 601-1326 Jpn
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Kitamoto K
Univ. Tokyo Tokyo Jpn
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Kitamoto K
National Research Institute Of Brewing
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Kitamoto Katsuhiko
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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MARUYAMA Jun-ichi
Department of Biotechnology, The University of Tokyo
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KADOKURA HIROSHI
Department of Biotechnology, The University of Tokyo
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Maruyama J
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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NAKAJIMA Harushi
Department of Biotechnology, The University of Tokyo
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OHNUMA HITOSHI
Japanese Red Cross Saitama Blood Center
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YOSHIKAWA AKIRA
Japanese Red Cross Saitama Blood Center
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Kitamoto Katsuhiko
Department Of Biotechnology University Of Tokyo
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Maruyama Jun-ichi
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Kadokura Hiroshi
Department Of Biotechnology The University Of Tokyo
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Kitamoto Katsuhiko
National Research Institute Of Brewing:(present Address)department Of Biotechnology The University O
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Nakajima H
Department Of Endocrine Breast Surgery Kyoto Prefectural University Of Medicine
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Kitamoto Katsuhiko
Department Of Applied Biological Chemistry And Department Of Applied Biological Engineering Graduate
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Ohnuma Hitoshi
Japanese Red Cross (jrc) Saitama Blood Center
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Maruyama Jun-ichi
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Nakajima Harushi
Department Of Agricultural Chemistry The University Of Tokyo
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Nakajima Hidenori
Exploratory Research Laboratories Fujisawa Pharmaceutical Co. Ltd
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Kimura K
Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokkaido University
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Maruyama Junichi
Department Of Biotechnology The University Of Tokyo
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Yoshikawa Akira
Japanese Red Cross (jrc) Saitama Blood Center
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