Insertion Analysis of Putative Functional Elements in the Promoter Region of the Aspergillus oryzae Taka-amylase A Gene (amyB) Using a Heterologous Aspergillus nidulans amdS-lacZ Fusion Gene System
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概要
- 論文の詳細を見る
Expression of the Taka-amylase A gene (amyB) of Aspergillus oryzae is induced by starch or maltose. The A. oryzae amyB gene promoter contains three highly conserved sequences, designated Regions I, II, and III, compared with promoter regions of the A. oryzae glaA encoding glucoamylase and the agdA encoding α-glucosidase. To identify the function of these sequences within the amyB promoter, various fragments containing conserved sequences in the amyB promoter were introduced into the upstream region of the heterologous A. nidulans amdS gene (encoding acetamidase) fused to the Escherichia coli lacZ gene as a reporter. Introduction of the sequence between -290 to -233 (the number indicates the distance in base pairs from the translation initiation point (+1)) containing Region III significantly increased the expression of the lacZ reporter gene in the presence of maltose. The sequence between -377 to -290 containing Region I also increased the lacZ activity, but its maltose inducibility was less than that of Region III. The sequence between -233 to -181 containing Region II had no effect on the expression. These results indicated that Region III is most likely involved in the maltose induction of the amyB gene expression.
- 社団法人日本農芸化学会の論文
- 1999-01-23
著者
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Kitamoto K
Univ. Tokyo Tokyo Jpn
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Kitamoto K
National Research Institute Of Brewing
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Kitamoto Katsuhiko
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Tamura G
Department Of Agricultural Chemistry The University Of Tokyo
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Tamura G
Research Institute Of Brewing Resources Co. Ltd.
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Tamura Gakuzo
Research Institute Of Brewing Resources Co. Ltd.
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Kitamoto Katsuhiko
Department Of Biotechnology University Of Tokyo
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Kitamoto Katsuhiko
National Research Institute Of Brewing
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GOMI Katsuya
National Research Institute of Brewing
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Tamura Gakuzo
Nuclear Receptor Institute Ksp
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KANEMORI Yoji
Research Institute of Gekkeikan Sake Co., Ltd.
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KUMAGAI Chieko
National Research Institute of Brewing
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Kitamoto Katsuhiko
National Research Institute Of Brewing:(present Address)department Of Biotechnology The University O
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Kumagai C
National Research Institute Of Brewing
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Gomi K
Tohoku Univ. Miyagi Jpn
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Kanemori Yoji
Research Institute Of Gekkeikan Sake Co. Ltd.
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Kumagai Chieko
General Research Laboratories Ozeki Corp.
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Kimura K
Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokkaido University
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