L-Canavanine Resistance as a Positive Selectable Marker in Diploid Yeast Transformation through Integral Disruption of the CAN1 Gene
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概要
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We have developed a novel transformation system for yeasts carrying no selectable markers. By using a plasmid, pYHH-1, containing an internal region of the CAN1 gene, a wild type yeast strain was endowed with L-canavanine resistance (canl) through integral disruption of the resident CAN1 gene. Diploid strains as well as haploids can be transformed, indicating that L-canavanine resistance can be used as a positive selectable marker for the transformation of industrial yeasts.
著者
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Iimura Yuzuru
National Research Institute of Brewing
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GOMI Katsuya
National Research Institute of Brewing
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TAKAHASHI Kojiro
National Research Institute of Brewing
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SUIZU Tetsuyoshi
Research Institute of Brewing Resources, Co., Ltd.
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YOSHIZAWA Kiyoshi
National Research Institute of Brewing
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Suizu Tetsuyoshi
Research Institute Gekkeikan Sake Co. Ltd.
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KARA Shodo
National Research Institute of Brewing
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