High Level Secretion of Calf Chymosin Using a Glucoamylase-prochymosin Fusion Gene in Aspergillus oryzae
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概要
- 論文の詳細を見る
A recombinant chymosin was secreted at high levels using fusion genes with A. oryzae glucoamylase gene (glaA) and a wheat bran solid-state culture system. Two portions of the A. oryzae glucoamylase, one with almost the entire glucoamylase (GA1-603) lacking 9 amino acids at the carboxyl terminal, and the other (GA1-511) lacking the starch binding-domain, were fused in frame with prochymosin cDNA. Western blot analysis indicated that the mature chymosin was released from the secreted fusion protein by autocatalytic processing. The transformant harboring the GA1-511-prochymosin construct showed about 5-fold chymosin production of the transformant in which the chymosin gene was directly expressed under the control of the glaA promoter in submerged culture. Moreover, wheat bran solid-state culture gave about 500-fold higher yield of the chymosin (approximately 150 mg/kg wheat bran) compared with the submerged culture.
- 社団法人日本農芸化学会の論文
- 1994-05-23
著者
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Kitamoto K
Univ. Tokyo Tokyo Jpn
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Kitamoto K
National Research Institute Of Brewing
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Tamura G
Department Of Agricultural Chemistry The University Of Tokyo
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Tamura G
Research Institute Of Brewing Resources Co. Ltd.
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Tamura Gakuzo
Research Institute Of Brewing Resources Co. Ltd.
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Kitamoto Katsuhiko
Department Of Biotechnology University Of Tokyo
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Kitamoto Katsuhiko
National Research Institute Of Brewing
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GOMI Katsuya
National Research Institute of Brewing
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Tamura Gakuzo
Nuclear Receptor Institute Ksp
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KUMAGAI Chieko
National Research Institute of Brewing
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Tsuchiya Kozo
Research Institute of Brewing Resources Co.
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Nagashima Tadashi
Shin Nihon Chemical Co., Ltd.
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Yamamoto Yutaka
Shin Nihon Chemical Co., Ltd.
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Nagashima T
Department Of Neurology Seiwa Memorial Hospital
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Nagashima Tadashi
National Research Institute Of Brewing:(present Address)technical Service Shin Nihon Chemical Co. Lt
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Nagashima Tadashi
Shin Nihon Chemical Co. Ltd
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Kumagai C
National Research Institute Of Brewing
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Gomi K
Tohoku Univ. Miyagi Jpn
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Kumagai Chieko
General Research Laboratories Ozeki Corp.
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Nagashima T
Teikyo Univ. School Of Medicine Ichihara Hospital
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Tsuchiya K
Nisshin Oil Mills Ltd. Yokohama‐shi Jpn
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Yamamoto Y
Faculty Of Bio-resource Sciences Akita Prefectural University
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Kimura K
Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokkaido University
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