Cloning and Nucleotide Sequence of the Calmodulin-Encoding Gene (cmdA) from Aspergillus oryzae
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概要
- 論文の詳細を見る
A cDNA and genomic gene encoding calmodulin were isolated from Aspergillus oryzae using a part of the calmodulin gene from A. nidulans as a hybridization probe. The gene was in a 3.4-kb SphI fragment and Southern-blot analysis of genomic DNA suggested the existence of a single copy of the calmodulin gene in A. oryzae. The nucleotide sequence analysis showed that the gene consists of five introns and six exons. Although the nucleotide sequence homology with that of A. nidulans was not so high (68%), the deduced amino acid sequence was 100% and 84% identical with calmodulin of A. nidulans and chicken, respectively. The cDNA encoding A. oryzae calmodulin was expressed under the control of the GAL1 promoter in the calmodulin null mutant (cmd1) of yeast, Saccharomyces cerevisiae, and could function as a calmodulin gene.
- 社団法人日本農芸化学会の論文
- 1995-08-23
著者
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Kitamoto K
Univ. Tokyo Tokyo Jpn
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Kitamoto K
National Research Institute Of Brewing
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Tamura G
Department Of Agricultural Chemistry The University Of Tokyo
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Tamura G
Research Institute Of Brewing Resources Co. Ltd.
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Tamura Gakuzo
Research Institute Of Brewing Resources Co. Ltd.
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Kitamoto Katsuhiko
Department Of Biotechnology University Of Tokyo
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Kitamoto Katsuhiko
National Research Institute Of Brewing
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GOMI Katsuya
National Research Institute of Brewing
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Tamura Gakuzo
Nuclear Receptor Institute Ksp
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KUMAGAI Chieko
National Research Institute of Brewing
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Yasui Katsuhiro
Research Institute of Brewing Resources, Co., Ltd.
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Ohya Yoshikazu
Department of Plant Sciences, Graduate School of Science, The University of Tokyo
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Kumagai C
National Research Institute Of Brewing
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Gomi K
Tohoku Univ. Miyagi Jpn
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Ohya Y
Univ. Tokyo Chiba Prefecture Jpn
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Ohya Yoshikazu
Department Of Biological Sciences Graduate School Of Science University Of To
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Kumagai Chieko
General Research Laboratories Ozeki Corp.
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Yasui Katsuhiro
Research Institute Gekkeikan Sake Co. Ltd.
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Kimura K
Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokkaido University
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