Improvement of Heterologous Protein Production in Aspergillus oryzae by RNA Interference with α-Amylase Genes
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概要
- 論文の詳細を見る
Aspergillus oryzae RIB40 has three α-amylase genes (amyA, amyB, and amyC), and secretes α-amylase abundantly. However, large amounts of endogenous secretory proteins such as α-amylase can compete with heterologous protein in the secretory pathway and decrease its production yields. In this study, we examined the effects of suppression of α-amylase on heterologous protein production in A. oryzae, using the bovine chymosin (CHY) as a reporter heterologous protein. The three α-amylase genes in A. oryzae have nearly identical DNA sequences from those promoters to the coding regions. Hence we performed silencing of α-amylase genes by RNA interference (RNAi) in the A. oryzae CHY producing strain. The silenced strains exhibited a reduction in α-amylase activity and an increase in CHY production in the culture medium. This result suggests that suppression of α-amylase is effective in heterologous protein production in A. oryzae.
- 社団法人 日本農芸化学会の論文
- 2009-11-23
著者
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Kitamoto Katsuhiko
Department of Biotechnology, The University of Tokyo
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Kitamoto K
Univ. Tokyo Tokyo Jpn
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Kitamoto Katsuhiko
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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MARUYAMA Jun-ichi
Department of Biotechnology, The University of Tokyo
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Kitamoto Katsuhiko
Department Of Biotechnology University Of Tokyo
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Nemoto Takashi
Department of Biotechnology, The University of Tokyo
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Kitamoto Katsuhiko
National Research Institute Of Brewing:(present Address)department Of Biotechnology The University O
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Kitamoto Katsuhiko
Department Of Applied Biological Chemistry And Department Of Applied Biological Engineering Graduate
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Maruyama Jun-ichi
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Nemoto Takashi
Department Of Biotechnology The University Of Tokyo
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