A Novel Culture Method for High Level Production of Heterologous Protein in Saccharomyces cerevisiae
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概要
- 論文の詳細を見る
A high level production system for heterologous protein by cold culture of yeast transformants at 15℃ was developed. The yeast transformants, carrying a plasmid containing cDNA for Aspergillus oryzae α-amylase (Taka-amylase A) or human lysozyme synthetic DNA, were cultivated in a selective medium for 1 or 2 days until full growth at 30℃. The yeast cells were harvested by centrifugation from the culture fluid and then were transferred to YPD medium. These inoculated broths were incubated for 2 days at 15℃ and then for another 2 days at 30℃. By the cold culture method described above, higher amounts of Taka-amylase A (28. 6 mg/liter) and human lysozyme (6. 1 mg/liter) were produced by the yeast transformants compared to those by conventional methods. Heterologous protein productions using YEp, YCp, and YIp types of yeast expression vectors with ADH1 or GAPDH promoter by the cold culture method showed effective productivity of about 2-fold compared to those by the conventional method of culture at 30℃. The high level production of heterologous protein by this method was not specific to the S. cerevisiae strains examined.
- 社団法人日本農芸化学会の論文
- 1994-07-23
著者
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Kitamoto K
Univ. Tokyo Tokyo Jpn
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Kitamoto K
National Research Institute Of Brewing
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Kitamoto Katsuhiko
Department Of Biotechnology University Of Tokyo
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Kitamoto Katsuhiko
National Research Institute Of Brewing
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GOMI Katsuya
National Research Institute of Brewing
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KUMAGAI Chieko
National Research Institute of Brewing
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Nagashima Tadashi
Shin Nihon Chemical Co., Ltd.
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Yamamoto Yutaka
Shin Nihon Chemical Co., Ltd.
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Nagashima T
Department Of Neurology Seiwa Memorial Hospital
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Nagashima Tadashi
National Research Institute Of Brewing:(present Address)technical Service Shin Nihon Chemical Co. Lt
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Nagashima Tadashi
Shin Nihon Chemical Co. Ltd
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Kumagai C
National Research Institute Of Brewing
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Gomi K
Tohoku Univ. Miyagi Jpn
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Kumagai Chieko
General Research Laboratories Ozeki Corp.
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Nagashima T
Teikyo Univ. School Of Medicine Ichihara Hospital
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Yamamoto Y
Faculty Of Bio-resource Sciences Akita Prefectural University
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Kimura K
Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokkaido University
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