Specific RNA Interference in psbP Genes Encoded by a Multigene Family in Nicotiana tabacum with a Short 3'-Untranslated Sequence(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
RNA interference with double-stranded RNA is a new method for the study of gene function in various organisms. In this report, we show that an inverted repeat of a short (103-bp) 3'-untranslated sequence of an isogene, 1A, of psbP genes, encoded by a small multigene family of four genes (1A, 2AF, 3F, and 5B) in Nicotiana tabacum, can specifically suppress the expression of psbP isogenes 1A and 5B with a 3'-untranslated sequence similar to a transcribed double-stranded RNA. The expression of other psbP isogenes, 2AF and 3F, was not affected, although the coding sequences of the psbP family genes are highly conserved. Consistent with this observation, small interfering RNAs were detected for the 3'-untranslated sequence used for the invertedrepeat transgene, and not for the coding sequence. These results suggest that double-stranded RNA having a 3'-untranslated sequence could be useful for an isogene-specific RNA interference of the family genes in Nicotiana tabacum.
- 社団法人日本農芸化学会の論文
- 2003-01-23
著者
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Sato Fumihiko
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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IFUKU Kentaro
Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University
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Ifuku K
Graduate School Of Biostudies Kyoto University
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Sato F
Div. Of Integrated Life Sci. Graduate School Of Biostudies Kyoto Univ.
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Sato F
Division Of Integrated Life Science Graduate School Of Biostudies Kyoto University
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YAMAMOTO Yumiko
Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University
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Ifuku Kentaro
Division Of Integrated Life Science Graduate School Of Biostudies Kyoto University
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Sato Fumihiko
Graduate School Of Biostudies Kyoto University
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Yamamoto Y
Division Of Integrated Life Science Graduate School Of Biostudies Kyoto University
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Yamamoto Y
Faculty Of Bio-resource Sciences Akita Prefectural University
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Sato Fumihiko
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Sato Fumihiko
Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University
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